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VCAM-1 secreted from cancer-associated fibroblasts enhances the growth and invasion of lung cancer cells through AKT and MAPK signaling.

癌相关成纤维细胞分泌的 VCAM-1 通过 AKT 和 MAPK 信号增强肺癌细胞的生长和侵袭。

  • 影响因子:6.50
  • DOI:10.1016/j.canlet.2019.12.039
  • 作者列表:"Zhou Z","Zhou Q","Wu X","Xu S","Hu X","Tao X","Li B","Peng J","Li D","Shen L","Cao Y","Yang L
  • 发表时间:2020-03-31
Abstract

:Several studies have indicated that cancer-associated fibroblasts (CAFs) could promote cancer progression in many malignancies. However, the mechanism by which CAFs promote the growth and metastasis of lung cancer remains poorly defined. In the present study, CAFs and normal fibroblasts (NFs) were isolated from human lung cancer and adjacent tissue. The data showed that the conditional medium (CM) of CAFs could increase the proliferation, migration and invasion of lung cancer cells. Vascular cell adhesion molecule-1 (VCAM-1) showed a higher expression in CAF-CM than NF-CM, and blocking VCAM-1 in CAF-CM attenuated the proliferation and invasion of cancer cells. Further, the results showed that VCAM-1 secreted from CAFs activated AKT and MAPK signaling via receptor α4β1 integrin (very-late antigen (VLA)-4) in lung cancer cells. Moreover, CAFs promoted VCAM-1 expression and tumor growth in vivo. Additionally, bioinformatics analysis indicated a positive correlation on the CAF marker protein alpha-smooth muscle actin (α-SMA) and VCAM-1 expression, which was associated with a poor prognosis in lung cancer patients. These findings demonstrate that the VCAM-1 secreted from CAFs enhances growth and invasion by activating the AKT and MAPK signaling of lung cancer cells.

摘要

: 一些研究表明,癌症相关成纤维细胞 (CAFs) 可以促进许多恶性肿瘤的癌症进展。然而,CAFs 促进肺癌生长和转移的机制仍然知之甚少。本研究从人肺癌和癌旁组织中分离出 CAFs 和正常成纤维细胞 (NFs)。数据表明,CAFs 的条件培养基 (CM) 可增加肺癌细胞的增殖、迁移和侵袭能力。血管细胞黏附分子-1 (VCAM-1) 在 CAF-CM 中的表达高于 NF-CM,阻断 CAF-CM 中的 VCAM-1 可减弱癌细胞的增殖和侵袭。结果表明,在肺癌细胞中,CAFs 分泌的 VCAM-1 通过受体 α 4 β 1 整合素 (very-late antigen,VLA)-4 激活 AKT 和 MAPK 信号。此外,CAFs 促进体内 VCAM-1 表达和肿瘤生长。此外,生物信息学分析表明 CAF 标记蛋白 α-平滑肌肌动蛋白 (α-SMA) 和 VCAM-1 的表达呈正相关,与肺癌患者的不良预后相关。这些发现证明 CAFs 分泌的 VCAM-1 通过激活肺癌细胞的 AKT 和 MAPK 信号来增强生长和侵袭。

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翻译标题与摘要 下载文献
影响因子:6.50
发表时间:2020-03-31
来源期刊:Cancer letters
DOI:10.1016/j.canlet.2019.12.039
作者列表:["Zhou Z","Zhou Q","Wu X","Xu S","Hu X","Tao X","Li B","Peng J","Li D","Shen L","Cao Y","Yang L"]

METHODS::Several studies have indicated that cancer-associated fibroblasts (CAFs) could promote cancer progression in many malignancies. However, the mechanism by which CAFs promote the growth and metastasis of lung cancer remains poorly defined. In the present study, CAFs and normal fibroblasts (NFs) were isolated from human lung cancer and adjacent tissue. The data showed that the conditional medium (CM) of CAFs could increase the proliferation, migration and invasion of lung cancer cells. Vascular cell adhesion molecule-1 (VCAM-1) showed a higher expression in CAF-CM than NF-CM, and blocking VCAM-1 in CAF-CM attenuated the proliferation and invasion of cancer cells. Further, the results showed that VCAM-1 secreted from CAFs activated AKT and MAPK signaling via receptor α4β1 integrin (very-late antigen (VLA)-4) in lung cancer cells. Moreover, CAFs promoted VCAM-1 expression and tumor growth in vivo. Additionally, bioinformatics analysis indicated a positive correlation on the CAF marker protein alpha-smooth muscle actin (α-SMA) and VCAM-1 expression, which was associated with a poor prognosis in lung cancer patients. These findings demonstrate that the VCAM-1 secreted from CAFs enhances growth and invasion by activating the AKT and MAPK signaling of lung cancer cells.

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