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Upregulation of lncRNA ZFAS1 promotes lung adenocarcinoma progression by sponging miR-1271-5p and upregulating FRS2.

LncRNA ZFAS1 的上调通过海绵 miR-1271-5p 和上调 frs2 促进肺腺癌进展。

  • 影响因子:2.17
  • DOI:10.1111/1759-7714.13525
  • 作者列表:"Fan G","Jiao J","Shen F","Chu F
  • 发表时间:2020-06-08
Abstract

BACKGROUND:Nowadays, the important roles of long non-coding RNAs (LncRNAs) in lung adenocarcinoma (LAD) is being increasingly recognized. The purpose of this study was to explore the regulatory mechanism of lncRNA ZFAS1 in LAD. METHODS:The expression and function of lncRNA ZFAS1 were assessed by RT-qPCR, CCK-8, transwell and dual luciferase reporter assays. RESULTS:Upregulation of lncRNA ZFAS1 was found in LAD tissues and cells. Knockdown of lncRNA ZFAS1 restrained cell proliferation, migration and invasion in LAD cells. In addition, we determined that lncRNA ZFAS1 could directly bind to miR-1271-5p. MiR-1271-5p functioned as a tumor suppressor in LAD, and lncRNA ZFAS1 promoted LAD development by downregulating miR-1271-5p. Furthermore, FRS2 was a direct target of miR-1271-5p. FRS2 promoted progression of LAD by mediating lncRNA ZFAS1/miR-1271-5p axis. CONCLUSIONS:LncRNA ZFAS1 promotes cell proliferation, migration and invasion in LAD by downregulating miR-1271-5p or upregulating FRS2.

摘要

背景: 目前人们越来越认识到长链非编码 rna (LncRNAs) 在肺腺癌 (LAD) 中的重要作用。本研究旨在探讨 lncRNA ZFAS1 在 LAD 中的调控机制。 方法: 采用 RT-qPCR 、 CCK-8 、 transwell 和双荧光素酶报告基因检测 lncRNA ZFAS1 的表达和功能。 结果: 在 LAD 组织和细胞中发现 lncRNA ZFAS1 上调。敲除 lncRNA ZFAS1 可抑制 LAD 细胞的增殖、迁移和侵袭。此外,我们确定 lncRNA ZFAS1 可以直接与 miR-1271-5p 结合。MiR-1271-5p 在 LAD 中起着肿瘤抑制因子的作用,lncRNA ZFAS1 通过下调 miR-1271-5p 促进 LAD 的发展。此外,FRS2 是 miR-1271-5p 的直接目标。FRS2 通过介导 lncRNA ZFAS1/miR-1271-5p 轴促进 LAD 进展。 结论: LncRNA ZFAS1 通过下调 miR-1271-5p 或上调 frs2 促进 LAD 细胞增殖、迁移和侵袭。

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作者列表:["Esme H","Can A","Şehitogullari A"]

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影响因子:1.84
发表时间:2020-01-01
来源期刊:Oncology letters
DOI:10.3892/ol.2019.11149
作者列表:["Das SK","Huang YY","Li B","Yu XX","Xiao RH","Yang HF"]

METHODS::The aim of the present study was to compare the safety and efficacy of cryoablation (CA) and microwave ablation (MWA) as treatments for non-small cell lung cancer (NSCLC). Patients with stage IIIB or IV NSCLC treated with CA (n=45) or MWA (n=56) were enrolled in the present study. The primary endpoint was progression-free survival (PFS); the secondary endpoints included overall survival (OS) time and adverse events (AEs). The median PFS times between the two groups were not significantly different (P=0.36): CA, 10 months [95% confidence interval (CI), 7.5-12.4] vs. MWA, 11 months (95% CI, 9.5-12.4). The OS times between the two groups were also not significantly different (P=0.07): CA, 27.5 months (95% CI, 22.8-31.2 months) vs. MWA, 18 months (95% CI, 12.5-23.5). For larger tumors (>3 cm), patients treated with MWA had significantly longer median PFS (P=0.04; MWA, 10.5 months vs. CA, 7.0 months) and OS times (P=0.04; MWA, 24.5 months vs. CA, 14.5 months) compared patients treated with CA. However, for smaller tumors (≤3 cm), median PFS (P=0.79; MWA, 11.0 months vs. CA, 13.0 months) and OS times (P=0.39; MWA, 30.0 months vs. CA, 26.5 months) between the two groups did not differ significantly. The incidence rates of AEs were similar in the two groups (P>0.05). The number of applicators, tumor size and length of the lung traversed by applicators were associated with a higher risk of pneumothorax and intra-pulmonary hemorrhage in the two groups. Treatment with CA resulted in significantly less intraprocedural pain compared with treatment with MWA (P=0.001). Overall, the present study demonstrated that CA and MWA were comparably safe and effective procedures for the treatment of small tumors. However, treatment with MWA was superior compared with CA for the treatment of large tumors.

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影响因子:8.44
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DOI:10.1016/j.annonc.2019.10.022
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