鼻咽癌 PET/MR 图像稳健影像特征的提取与选择
- 作者列表："Yang, Pengfei","Xu, Lei","Cao, Zuozhen","Wan, Yidong","Xue, Yi","Jiang, Yangkang","Yen, Eric","Luo, Chen","Wang, Jing","Rong, Yi","Niu, Tianye
Objectives This work aims to study the variation, robustness, and feature redundancy of PET/MR radiomic features in the primary tumor of nasopharyngeal carcinoma (NPC). Procedures PET/MR scans of 21 NPC patients were used in this study. The primary tumor volumes were defined using PET, T2-weighted-MR (T2-MR), and diffusion-weighted MR (DW-MR) images. A random-dilation-erosion method was used to simulate 10 sets of tumor volumes for identifying features invariant with manual segmentation uncertainties. Feature robustness was evaluated against imaging modalities, pixel sizes, slice thickness, and grey-level bin sizes using intraclass correlation coefficient (ICC) and spearman correlation coefficient. Feature redundancy was analyzed using the hierarchical cluster analysis. Results Voxel size of 0.5 × 0.5 × 1.0 mm^3 was found optimal for robust feature extraction from PET and MR. Normalized grey level of 64 and 128 was suggested for PET and MR, respectively. The features from wavelet-transformed images were less stable than those from the original images. The robustness analysis and volume correlation analysis identified 335 (62.04 %) PET features, 240 (44.44 %) T2-MR features, and 366 (67.78 %) DW-MR features. The cluster analysis grouped PET, T2-MR, and DW-MR features into 106, 83, and 133 representative features, respectively. Conclusions The present study analyzed and identified robust features extracted from tumor volumes on PET/MR, which can provide guidance and promote standardization for PET/MR radiomic studies in NPC.
目的研究鼻咽癌原发灶 PET/MR 影像组学特征的变异、鲁棒性和特征冗余。本研究采用了 21 例鼻咽癌患者的 PET/MR 扫描。使用 PET 、 T2-weighted-MR (T2-MR) 和扩散加权 MR (DW-MR) 图像定义原发肿瘤体积。采用随机-扩张-侵蚀方法模拟 10 组肿瘤体积，用于识别具有手动分割不确定性的不变特征。使用组内相关系数 (ICC) 和 spearman 相关系数，针对成像模式、像素大小、切片厚度和灰度 bin 大小评估特征稳健性。使用分层聚类分析分析特征冗余。结果对于 PET 和 MR 的稳健特征提取，体素大小为 0.5 × 1.0毫米 ^ 3 是最佳的。对于 PET 和 MR，建议归一化灰度分别为 64 和 128。小波变换图像的特征比原始图像的特征稳定性差。稳健性分析和体积相关分析确定了 335 (62.04%) 个 PET 特征、 240 (44.44%) 个 T2-MR 特征和 366 (67.78%) 个 DW-MR 特征。聚类分析将 PET 、 T2-MR 和 DW-MR 特征分别分为 106 、 83 和 133 个代表性特征。结论本研究分析并识别了 PET/MR 上肿瘤体积提取的稳健特征，可为鼻咽癌 PET/MR 影像组学研究提供指导和促进标准化。
METHODS:INTRODUCTION:Human papillomavirus (HPV) is the most common sexually transmitted infection and is associated with several types of cancer. The number of cases of HPV-associated head and neck squamous cell carcinomas (HNSCCs), especially oropharyngeal carcinomas, has increased significantly in recent years despite decreased tobacco smoking rates. Currently, no data concerning the risk factors and prevalence of HPV in HNSCC patients in all regions of Brazil are available, making it difficult to promote advances in this field of public health. Therefore, our goal is to determine the impact of infection by HPV, including HPVs with different genotypes, on head and neck cancer and the risk factors associated with the development of head and neck cancer in all regions of Brazil. METHODS AND ANALYSIS:This is a case-control study that will include 622 patients and 622 controls from all regions of Brazil. A questionnaire will be applied to gather information on sociodemographic, behavioural and health factors. Oral, cervical or penile/scrotal, and anal specimens and serum samples will be collected from all participants. Formalin-fixed paraffin-embedded tissue from tumour biopsies will be analysed only in the case group. Molecular and serological analyses will be performed to evaluate the presence and role of HPV in the development of head and neck cancer. ETHICS AND DISSEMINATION:This project was approved by the research ethical committee of the proposing institution (Hospital Moinhos de Vento, number 2.852.060). Ethical approval from the collaborators is currently under evaluation and is not yet complete. The results of this study will be presented at meetings with the Brazilian Ministry of Health through technical reports and to the scientific community at national and international events, with subsequent publication of scientific articles.
METHODS:BACKGROUND:Factors related to head and neck cancer and the treatment of the disease can affect quality of life. The aim of this study was to determine factors associated with the severity of impact on oral health-related quality of life (OHRQoL) in survivors of head and neck cancer using a multivariate analysis. METHODS:This cross-sectional study evaluated 90 volunteers who had completed radiotherapy at least 3 months earlier. OHRQoL was assessed using oral health impact profile (OHIP-14) and the data were analyzed using robust variance poisson regression models. RESULTS:The mean total OHIP-14 score was 23.98 ± 12.55. Patients with hyposalivation had 56% higher (worse) mean OHIP-14 total scores (CI:1.11-2.18) and patients with advanced stage tumors had 31% higher mean OHIP-14 total scores (CI:1.03-1.66) in multivariate analyses. CONCLUSION:OHRQoL of survivors of head and neck cancer experienced a negative impact following radiotherapy. The impact was associated with hyposalivation and advanced stage tumors.
METHODS:BACKGROUND:To immunohistochemically evaluate the association between the presence of cancer-associated fibroblasts (CAFs) and the tumour expression of podoplanin (PDPN) in head and neck squamous cell carcinoma (HNSCC) and their association with clinicopathological variables. MATERIAL AND METHODS:A tissue microarray (TMA) with biopsy sections from patients diagnosed with HNSCC was stained with antibodies against the CAFs marker, α-smooth muscle actin (α-SMA), and PDPN. We subsequently evaluated their expression to determine the association between them and with clinicopathological variables including age, primary tumour site, TNM stage, and tumour differentiation grade. RESULTS:Positive reaction to α-SMA was observed in the tumour stroma, revealing spindle-shaped cells compatible with CAFs, which showed a high expression in 62% of cases and a significant association with laryngeal carcinomas, advanced clinical stages, and lower tumour differentiation (P ≤ 0.05). PDPN staining on tumour cells showed low expression in 72% of cases, and it was not associated with any clinicopathological variable or with the presence of CAFs. CONCLUSIONS:The presence of CAFs in the tumour stroma is related to an aggressive phenotype and could increase as the disease progresses, although based on our findings, it would have no relationship, at least directly, with the expression of PDPN.