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Increased CSF levels of apolipoproteins and complement factors in trigeminal neuralgia patients - in depth proteomic analysis using mass spectrometry.

三叉神经痛患者载脂蛋白和补体因子 CSF 水平升高-使用质谱进行深入的蛋白质组学分析。

  • 影响因子:5.15
  • DOI:10.1016/j.jpain.2020.03.002
  • 作者列表:"Hamdeh SA","Khoonsari PE","Shevchenko G","Gordh T","Ericson H","Kultima K
  • 发表时间:2020-06-14
Abstract

:The main cause of trigeminal neuralgia (TN) is compression of a blood vessel at the root entry zone of the trigeminal nerve. However, a neurovascular conflict does not seem to be the only etiology and other mechanisms are implicated in the development of the disease. We hypothesized that TN patients may have distinct protein expression in the CSF. In this study, lumbar CSF from TN patients (n=17), scheduled to undergo microvascular decompression (MVD), and from controls (n=20) was analyzed and compared with mass spectrometry-based shotgun proteomics. 2552 unique proteins were identified of which 46 were significantly altered (26 increased, and 20 decreased, q-value < 0.05) in TN patients compared with controls. An over-representation analysis showed proteins involved in high-density lipoprotein (HDL), such as APOA-4, APOM and APOA-1, and the extracellular region, including proteins involved in the complement cascade to be over-represented. We conclude that TN patients have distinct protein expression in the CSF compared to controls. The pathophysiological background of the protein alterations found in this study warrants further investigation in future studies. Perspective: In this article, cerebrospinal fluid from patients with trigeminal neuralgia was analyzed using in depth shotgun proteomics, revealing 46 differentially expressed proteins compared to controls. Among these, apolipoproteins and proteins involved in the complement system were elevated and significantly over-represented, implying an inflammatory component in the pathophysiology of the disease.

摘要

: 三叉神经痛 (TN) 的主要原因是三叉神经根进入区的血管受压。然而,神经血管冲突似乎不是唯一的病因,其他机制与疾病的发展有关。我们假设 TN 患者在 CSF 中可能有不同的蛋白表达。在这项研究中,来自 TN 患者 (n = 17) 的腰椎 CSF,计划接受微血管减压术 (MVD),以及来自对照组 (n = 20) 进行了分析,并与基于质谱的鸟枪法蛋白质组学进行了比较。鉴定出 2552 个独特的蛋白,其中 TN 患者与对照组相比,46 个显著改变 (26 个增加,20 个降低,q 值 <0.05)。过表达分析显示参与高密度脂蛋白 (HDL) 的蛋白质,如 APOA-4,APOM 和 APOA-1,以及细胞外区,包括参与补体级联反应的蛋白过度表达。我们得出结论,与对照组相比,TN 患者的 CSF 中有不同的蛋白表达。本研究中发现的蛋白质改变的病理生理背景值得在未来的研究中进一步研究。观点: 在这篇文章中,使用深入的鸟枪法蛋白质组学分析了三叉神经痛患者的脑脊液,揭示了与对照组相比的 46 个差异表达蛋白。其中,涉及补体系统的载脂蛋白和蛋白质升高并显著过度表达,这意味着疾病病理生理学中的炎症成分。

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