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Validation of Enhancing Effects of Curcumin on Radiotherapy with F98/FGT Glioblastoma-Bearing Rat Model

姜黄素对 F98/FGT 胶质母细胞瘤大鼠模型放疗增强作用的验证

  • 影响因子:4.32
  • DOI:10.3390/ijms21124385
  • 作者列表:"Wei-Hsun Wang","Chao-Yu Shen","Yi-Chun Chien","Wen-Shin Chang","Chia-Wen Tsai","Yi-Hsien Lin","Jeng-Jong Hwang
  • 发表时间:2020-06-20
Abstract

Glioblastoma, the most common and aggressive brain tumor with low survival rate, is difficult to be cured by neurosurgery or radiotherapy. Mounting evidence has reported the anti-inflammatory and anticancer effects of curcumin on several types of cancer in preclinical studies and clinical trials. To our knowledge, there is no platform or system that could be used to effectively and real-timely evaluate the therapeutic efficacy of curcumin for glioblastoma multiforme (GBM). In this study, we constructed a lentivirus vector with triple-reporter genes (<i>Fluc</i>/<i>GFP</i>/<i>tk</i>) and transduced into rat F98 glioblastoma cells to establish an orthotopic F98/<i>FGT</i> glioma-bearing rat model. In the model, the therapeutic efficacies for curcumin alone, radiation alone, and their combination were evaluated via noninvasive bioluminescent imaging and overall survival measurements. At the cell level, curcumin is capable of causing a G2/M cell cycle arrest and sensitizing the F98 cells to radiation. In animal model, curcumin synergistically enhances the effects of radiotherapy on suppressing the growth of both transplanted glioma cells and in situ brain tumors, and extending the overall survival periods longer than those of curcumin alone and radiation alone treatments. In conclusion, we have demonstrated that curcumin may serve as a novel radiosensitizer to combine with radiotherapy using the triple-reporter F98/<i>FGT</i> animal model for effective and simultaneous evaluation of therapeutic efficacy.

摘要

胶质母细胞瘤是最常见、最具侵袭性的脑肿瘤,生存率低,很难通过神经外科手术或放疗治愈。越来越多的证据报道了姜黄素在临床前研究和临床试验中对几种类型的癌症的抗炎和抗癌作用。据我们所知,目前尚无平台或系统可用于有效、实时评价姜黄素对多形性胶质母细胞瘤 (GBM) 的治疗效果。在这项研究中,我们构建了一个含有三个报告基因的慢病毒载体 (<i>Fluc</i>/<i>GFP</i>/<i>tk</i>) 并转导入大鼠 F98 胶质母细胞瘤细胞,建立原位 F98/<i>FGT</i> 荷胶质瘤大鼠模型。在模型中,通过无创生物发光成像和总生存测量评价姜黄素单独、单独放疗及其联合的治疗效果。在细胞水平上,姜黄素能够引起 G2/M 期细胞周期阻滞,并使 F98 细胞对辐射敏感。在动物模型中,姜黄素协同增强放疗对抑制移植胶质瘤细胞和原位脑肿瘤生长的作用,延长总生存期比单独使用姜黄素和放射治疗更长。总之,我们已经证明姜黄素可以作为一种新的放射增敏剂与放疗结合,使用三重报告 F98/<i>FGT</i> 动物模型来有效和同时评价治疗疗效。

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