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Therapeutic effects of vitamin D on acetic acid-induced colitis in rats.

维生素d 对乙酸诱导的大鼠结肠炎的治疗作用。

  • 影响因子:1.05
  • DOI:10.1590/s0102-865020200040000004
  • 作者列表:"Bademci R","Erdoğan MA","Kara AY","Yiğittürk G","Erbaş O
  • 发表时间:2020-06-05
Abstract

PURPOSE:To analyze the effect of calcitriol treatment on acute colitis in an experimental rat model. METHODS:A total of 24 adult Sprague Dawley albino rats were randomly separated into 3 equal groups: control group (n:8), colitis group (n:8), calcitriol administered group (n:8). A single dose of acetic acid (1 ml of 4% solution) was administered intrarectally to induce colitis. Group 1 was given 1 ml/kg 0.9% NaCl intraperitoneally; rats belonging to Group 2 were administered calcitriol 1 µg/kg for 5 days. RESULTS:Plasma tumor necrosis factor alpha, Pentraxin 3, and malondialdehyde levels were significantly lower in the calcitriol administered colitis group than in the standard colitis group (p<0.01). In the Calcitriol group, there was a significant histological improvement in hyperemia, hemorrhage and necrotic areas in the epithelium compared to the placebo group (p <0.000). CONCLUSION:The findings suggest that calcitriol may be an agent that could be used in acute colitis treatment.

摘要

目的: 分析骨化三醇对实验性大鼠急性结肠炎的治疗作用。 方法: 成年 sd 大鼠 24 只,随机分为 3 组: 对照组 (n:8) 、结肠炎组 (n:8) 、骨化三醇给药组 (n:8)。单剂量醋酸 (1毫升 ml 4% 溶液) 直肠内给药诱导结肠炎。组 1 腹腔给予 1毫升 ml/kg 0.9% NaCl; 组 2 大鼠给予骨化三醇 1 µ g/kg,连续 5 天。 结果: 血浆肿瘤坏死因子 α 、正五聚蛋白 3 和丙二醛水平在骨化三醇给药的结肠炎组明显低于标准结肠炎组 (p<0.01)。与安慰剂组相比,骨化三醇组上皮充血、出血和坏死区域的组织学改善显著 (p <0.000)。 结论: 骨化三醇可能是一种治疗急性结肠炎的药物。

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发表时间:2020-01-21
来源期刊:mBio
DOI:10.1128/mBio.03105-19
作者列表:["Kuehl CJ","D'Gama JD","Warr AR","Waldor MK"]

METHODS:Shigella species cause diarrheal disease globally. Shigellosis is typically characterized by bloody stools and colitis with mucosal damage and is the leading bacterial cause of diarrheal death worldwide. After the pathogen is orally ingested, it invades and replicates within the colonic epithelium through mechanisms that rely on its type III secretion system (T3SS). Currently, oral infection-based small animal models to study the pathogenesis of shigellosis are lacking. Here, we found that orogastric inoculation of infant rabbits with Shigella flexneri resulted in diarrhea and colonic pathology resembling that found in human shigellosis. Fasting animals prior to S. flexneri inoculation increased the frequency of disease. The pathogen colonized the colon, where both luminal and intraepithelial foci were observed. The intraepithelial foci likely arise through S. flexneri spreading from cell to cell. Robust S. flexneri intestinal colonization, invasion of the colonic epithelium, and epithelial sloughing all required the T3SS as well as IcsA, a factor required for bacterial spreading and adhesion in vitro Expression of the proinflammatory chemokine interleukin 8 (IL-8), detected with in situ mRNA labeling, was higher in animals infected with wild-type S. flexneri versus mutant strains deficient in icsA or T3SS, suggesting that epithelial invasion promotes expression of this chemokine. Collectively, our findings suggest that oral infection of infant rabbits offers a useful experimental model for studies of the pathogenesis of shigellosis and for testing of new therapeutics.IMPORTANCEShigella species are the leading bacterial cause of diarrheal death globally. The pathogen causes bacillary dysentery, a bloody diarrheal disease characterized by damage to the colonic mucosa and is usually spread through the fecal-oral route. Small animal models of shigellosis that rely on the oral route of infection are lacking. Here, we found that orogastric inoculation of infant rabbits with S. flexneri led to a diarrheal disease and colonic pathology reminiscent of human shigellosis. Diarrhea, intestinal colonization, and pathology in this model were dependent on the S. flexneri type III secretion system and IcsA, canonical Shigella virulence factors. Thus, oral infection of infant rabbits offers a feasible model to study the pathogenesis of shigellosis and to develop and test new therapeutics.

影响因子:5.36
发表时间:2020-01-20
DOI:10.1007/s00259-020-04686-1
作者列表:["Willowson KP","Schembri GP","Bernard EJ","Chan DL","Bailey DL"]

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影响因子:3.32
发表时间:2020-01-09
DOI:10.1016/j.intimp.2019.106144
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