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First isolation and whole-genome characterization of a G9P[14] rotavirus strain from a diarrheic child in Egypt.

埃及腹泻患儿 G9P[14] 轮状病毒株的隔离和全基因组特征。

  • 影响因子:2.78
  • DOI:10.1099/jgv.0.001455
  • 作者列表:"Shoeib A","Velasquez Portocarrero DE","Wang Y","Jiang B
  • 发表时间:2020-06-17
Abstract

An unusual group A rotavirus (RVA) strain (RVA/Human-tc/EGY/AS997/2012/G9[14]) was isolated for the first time in a faecal sample from a 6-month-old child who was hospitalized for treatment of acute gastroenteritis in Egypt in 2012. Whole-genome analysis showed that the strain AS997 had a unique genotype constellation: G9-P[14]-I2-R2-C2-M2-A11-N2-T1-E2-H1. Phylogenetic analysis indicated that the strain AS997 had the consensus P[14] genotype constellation with the G9, T1 and H1 reassortment. This suggests either a mixed gene configuration originated from a human Wa-like strain and a P[14]-containing animal virus, or that this P[14] could have been acquired via reassortment of human strains only. The study shows the possible roles of interspecies transmission and multiple reassortment events leading to the generation of novel rotavirus genotypes and underlines the importance of whole-genome characterization of rotavirus strains in surveillance studies.

摘要

不寻常的 A 组轮状病毒 (RVA) 株 (RVA/Human-tc/EGY/AS997/2012/G9[14]) 2012 年在埃及住院接受急性胃肠炎治疗的 6 个月大的儿童粪便样本中首次分离。全基因组分析表明,菌株 AS997 具有独特的基因型星座: G9-P[14]-I2-R2-C2-M2-A11-N2-T1-E2-H1。系统进化分析表明,菌株 AS997 具有 G9 、 T1 和 H1 重配的共有 P[14] 基因型星座。这表明混合基因构型起源于人类 Wa 样株和含 P[14] 的动物病毒,或者该 P[14] 可能是仅通过重配人类菌株获得的。该研究显示了种间传播和多次重配事件导致新型轮状病毒基因型产生的可能作用,并强调了轮状病毒菌株全基因组特征在监测研究中的重要性。

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作者列表:["Kuehl CJ","D'Gama JD","Warr AR","Waldor MK"]

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影响因子:5.36
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作者列表:["Willowson KP","Schembri GP","Bernard EJ","Chan DL","Bailey DL"]

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