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Overexpression of miR-199/214 is a distinctive feature of iron-induced and asbestos-induced sarcomatoid mesothelioma in rats.

MiR-199/214 的过度表达是大鼠中铁诱导和石棉诱导的肉瘤样间皮瘤的显著特征。

  • 影响因子:4.60
  • DOI:10.1111/cas.14405
  • 作者列表:"Okazaki Y","Chew SH","Nagai H","Yamashita Y","Ohara H","Jiang L","Akatsuka S","Takahashi T","Toyokuni S
  • 发表时间:2020-06-01
Abstract

:Malignant mesothelioma (MM) is one of the most lethal tumors in humans. The onset of MM is linked to exposure to asbestos, which generates reactive oxygen species (ROS). ROS are believed to be derived from the frustrated phagocytosis and the iron in asbestos. To explore the pathogenesis of MM, peritoneal MM was induced in rats by the repeated intraperitoneal injection of iron saccharate and nitrilotriacetate. In the present study, we used microarray techniques to screen the microRNA (miR) expression profiles of these MM. We observed that the histological subtype impacted the hierarchical clustering of miR expression profiles and determined that miR-199/214 is a distinctive feature of iron saccharate-induced sarcomatoid mesothelioma (SM). Twist1, a transcriptional regulator of the epithelial-mesenchymal transition, has been shown to activate miR-199/214 transcription; thus, the expression level of Twist1 was examined in iron-induced and asbestos-induced mesotheliomas in rats. Twist1 was exclusively expressed in iron saccharate-induced SM but not in the epithelioid subtype. The Twist1-miR-199/214 axis is activated in iron saccharate-induced and asbestos-induced SM. The expression levels of miR-214 and Twist1 were correlated in an asbestos-induced MM cell line, suggesting that the Twist1-miR-199/214 axis is preserved. MeT5A, an immortalized human mesothelial cell line, was used for the functional analysis of miR. The overexpression of miR-199/214 promoted cellular proliferation, mobility and phosphorylation of Akt and ERK in MeT5A cells. These results indicate that miR-199/214 may affect the aggressive biological behavior of SM.

摘要

: 恶性间皮瘤 (MM) 是人类最致命的肿瘤之一。MM的发病与暴露于石棉有关,石棉会产生活性氧 (ROS)。ROS被认为来源于受挫吞噬作用和石棉中的铁。为探讨MM的发病机制,采用多次腹腔注射糖精铁和次氮基三乙酸酯的方法诱导大鼠腹膜MM。在本研究中,我们使用微阵列技术筛选这些MM的microRNA (miR) 表达谱。我们观察到组织学亚型影响miR表达谱的分级聚类,并确定miR-199/214 是糖二酸铁诱导的肉瘤样间皮瘤 (SM) 的显著特征。Twist1 是上皮-间充质转化的转录调节因子,已被证明可激活miR-199/214 转录; 因此,在大鼠的铁诱导和石棉诱导的间皮瘤中检测Twist1 的表达水平。Twist1 仅在铁糖精诱导的SM中表达,而在上皮样亚型中不表达。Twist1-miR-199/214 轴在糖精铁诱导和石棉诱导的SM中被激活。MiR-214 和Twist1 的表达水平在石棉诱导的MM细胞系中相关,表明Twist1-miR-199/214 轴被保留。MeT5A是一种永生化的人间皮细胞系,用于miR的功能分析。MiR-199/214 的过表达促进MeT5A细胞的细胞增殖、迁移和Akt和ERK的磷酸化。这些结果表明miR-199/2 14 可能影响SM的侵袭性生物学行为。

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影响因子:6.93
发表时间:2020-01-15
DOI:10.1002/ijc.32532
作者列表:["Hata A","Nakajima T","Matsusaka K","Fukuyo M","Morimoto J","Yamamoto T","Sakairi Y","Rahmutulla B","Ota S","Wada H","Suzuki H","Matsubara H","Yoshino I","Kaneda A"]

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翻译标题与摘要 下载文献
影响因子:6.93
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DOI:10.1002/ijc.32530
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肺肿瘤方向

肺肿瘤,又叫支气管肺癌,是常见的恶性肿瘤之一。肺肿瘤的治疗为包括手术、中药、放疗、化疗及免疫等多学科的综合治疗。

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