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Effect of ethanol extracts of Antrodia cinnamomea on head and neck squamous cell carcinoma cell line.

牛樟芝乙醇提取物对头颈部鳞癌细胞的作用.

  • 影响因子:1.82
  • DOI:10.1590/1414-431x20208694
  • 作者列表:"Liu L","Wang C
  • 发表时间:2020-01-01
Abstract

:Head and neck squamous cell carcinoma (HNSCC) is one of the most common malignant tumors. Ethanol extract of Antrodia cinnamomea (EEA) has been widely studied for its health benefits including anticancer effects. The purpose of this study was to assess the effects of EEA on HNSCC. Cell proliferation, transwell, and wound healing assays were performed. The impact of EEA on tumor growth was investigated using a xenograft model. Expressions of migration-related proteins (MMP-2, MMP-9, TIMP-1, and TIMP-2) and apoptosis-related proteins (cleaved caspase-9 and cleaved PARP) were determined using western blot analysis. The results indicated that EEA significantly inhibited the capacities of proliferation, invasion, and migration of HNSCC cells in a dose-dependent manner. Cleaved caspase-9 and cleaved PARP expressions were increased in cells treated with an increasing concentration of EEA, which suggested that EEA induced apoptosis of HNSCC. MMP-2 and MMP-9 were downregulated when cells were administered EEA, while TIMP-1 and TIMP-2 were not affected, which uncovered the mechanisms mediating the EEA-induced inhibition on cell invasion and migration. The animal experiment also suggested that EEA inhibited tumor growth. Our study confirmed the inhibitive effects of EEA on cell proliferation, invasion, and migration of HNSCC in vitro and in vivo, providing the basis for further study of the application of EEA as an effective candidate for cancer treatment.

摘要

: 头颈部鳞状细胞癌 (HNSCC) 是最常见的恶性肿瘤之一。樟芝 (EEA) 的乙醇提取物已被广泛研究其健康益处,包括抗癌作用。本研究的目的是评估EEA对HNSCC的影响。进行细胞增殖、transwell和伤口愈合测定。使用异种移植模型研究EEA对肿瘤生长的影响。使用蛋白质印迹分析确定迁移相关蛋白 (MMP-2 、MMP-9 、TIMP-1 和TIMP-2) 和凋亡相关蛋白 (切割caspase-9 和切割PARP) 的表达。结果表明,EEA显著抑制HNSCC细胞的增殖、侵袭和迁移能力,且呈剂量依赖性。在用增加浓度的EEA处理的细胞中,切割的caspase-9 和切割的PARP表达增加,这表明EEA诱导HNSCC的凋亡。当给细胞施用EEA时,MMP-2 和MMP-9 下调,而TIMP-1 和TIMP-2 不受影响,这揭示了介导EEA诱导的对细胞侵袭和迁移的抑制的机制。动物实验也提示EEA抑制肿瘤生长。我们的研究证实了EEA在体外和体内对HNSCC细胞增殖、侵袭和迁移的抑制作用,为进一步研究EEA作为肿瘤治疗有效候选药物的应用提供依据。

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影响因子:6.93
发表时间:2020-01-15
DOI:10.1002/ijc.32532
作者列表:["Hata A","Nakajima T","Matsusaka K","Fukuyo M","Morimoto J","Yamamoto T","Sakairi Y","Rahmutulla B","Ota S","Wada H","Suzuki H","Matsubara H","Yoshino I","Kaneda A"]

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影响因子:6.93
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DOI:10.1002/ijc.32530
作者列表:["Zhang L","Yang Y","Chai L","Bu H","Yang Y","Huang H","Ran J","Zhu Y","Li L","Chen F","Li W"]

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肺肿瘤方向

肺肿瘤,又叫支气管肺癌,是常见的恶性肿瘤之一。肺肿瘤的治疗为包括手术、中药、放疗、化疗及免疫等多学科的综合治疗。

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