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White blood cell and cell-free DNA analyses for detection of residual disease in gastric cancer.

白细胞和无细胞 DNA 分析检测胃癌残留病。

  • 影响因子:12.19
  • DOI:10.1038/s41467-020-14310-3
  • 作者列表:"Leal A","van Grieken NCT","Palsgrove DN","Phallen J","Medina JE","Hruban C","Broeckaert MAM","Anagnostou V","Adleff V","Bruhm DC","Canzoniero JV","Fiksel J","Nordsmark M","Warmerdam FARM","Verheul HMW","van Spronsen DJ","Beerepoot LV","Geenen MM","Portielje JEA","Jansen EPM","van Sandick J","Meershoek-Klein Kranenbarg E","van Laarhoven HWM","van der Peet DL","van de Velde CJH","Verheij M","Fijneman R","Scharpf RB","Meijer GA","Cats A","Velculescu VE
  • 发表时间:2020-01-27
Abstract

:Liquid biopsies are providing new opportunities for detection of residual disease in cell-free DNA (cfDNA) after surgery but may be confounded through identification of alterations arising from clonal hematopoiesis. Here, we identify circulating tumor-derived DNA (ctDNA) alterations through ultrasensitive targeted sequencing analyses of matched cfDNA and white blood cells from the same patient. We apply this approach to analyze samples from patients in the CRITICS trial, a phase III randomized controlled study of perioperative treatment in patients with operable gastric cancer. After filtering alterations from matched white blood cells, the presence of ctDNA predicts recurrence when analyzed within nine weeks after preoperative treatment and after surgery in patients eligible for multimodal treatment. These analyses provide a facile method for distinguishing ctDNA from other cfDNA alterations and highlight the utility of ctDNA as a predictive biomarker of patient outcome to perioperative cancer therapy and surgical resection in patients with gastric cancer.

摘要

: 液体活检为术后无细胞 DNA (cfDNA) 中残留疾病的检测提供了新的机会,但可能通过鉴定克隆性造血引起的改变而混淆。在此,我们通过对同一患者匹配的 cfDNA 和白细胞进行超灵敏的靶向测序分析,确定循环肿瘤来源的 DNA (ctDNA) 改变。我们应用这种方法分析来自 criters 试验患者的样本,这是一项可手术胃癌患者围手术期治疗的 III 期随机对照研究。过滤匹配白细胞的改变后,ctDNA 的存在可预测术前治疗后 9 周内和术后符合多模式治疗条件的患者的复发。这些分析为区分 ctDNA 与其他 cfDNA 改变提供了一种简便的方法,并突出了 ctDNA 作为患者预后的预测生物标志物对胃癌患者围手术期癌症治疗和手术切除的效用。

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DOI:10.1002/mc.23158
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影响因子:1.71
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作者列表:["Jing JJ","Li H","Wang ZY","Zhou H","Sun LP","Yuan Y"]

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影响因子:3.89
发表时间:2020-01-28
DOI:10.1002/jcp.29562
作者列表:["Daryabari SS","Fathi M","Mahdavi M","Moaddab Y","Hosseinpour Feizi MA","Shokoohi B","Safaralizadeh R"]

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