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The First Quarter of SARS-CoV-2 Testing: the University of Washington Medicine Experience.

SARS-CoV-2第一季度检测: 华盛顿大学医学经验。

  • 影响因子:3.65
  • DOI:10.1128/JCM.01416-20
  • 作者列表:"Greninger AL","Jerome KR
  • 发表时间:2020-07-23
Abstract

:In early March 2020, the University of Washington Medical Center clinical virology laboratory became one of the first clinical laboratories to offer testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). When we first began test development in mid-January, neither of us believed there would be more than 2 million confirmed SARS-CoV-2 infections nationwide or that we would have performed more than 150,000 real-time PCR (RT-PCR) tests, with many more to come. This article will be a chronological summary of how we rapidly validated tests for SARS-CoV-2, increased our testing capacity, and addressed the many problems that came up along the way.

摘要

: 2020年3月初,华盛顿大学医学中心临床病毒学实验室成为首批提供新型冠状病毒检测的临床实验室之一 (SARS-CoV-2)。当我们在1月中旬第一次开始测试开发时,我们都不相信SARS-CoV-2全国范围内会有超过200万例确诊感染,也不相信我们会进行超过150,000例实时PCR (rt-pcr) 测试,还有更多。本文将按时间顺序总结我们如何快速验证SARS-CoV-2的测试,提高我们的测试能力,并解决在此过程中出现的许多问题。

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发表时间:2020-01-01
来源期刊:Acta cytologica
DOI:10.1159/000496568
作者列表:["Yu GH","Glaser LJ","Gustafson KS"]

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影响因子:0.85
发表时间:2020-01-02
来源期刊:Laboratory medicine
DOI:10.1093/labmed/lmz030
作者列表:["Yang R","Zhang R","Zhang Y","Huang Y","Liang H","Gui G","Gong S","Wang H","Xu M","Fan J"]

METHODS:OBJECTIVE:To assess the rate of, and risk factors for, human cytomegalovirus viremia (HCMV) in donor+/recipient+ (HCMV serostatus matched) hematopoietic stem-cell transplantation (HSCT) recipients. METHODS:HCMV DNA from 144 donor+/recipient+ HSCT recipients was examined by quantitative polymerase chain reaction (qPCR). RESULTS:The cumulative incidence of HCMV viremia was 69.4% (100/144) during the 48 weeks after HSCT. In a multivariate analysis, acute graft-versus-host disease (aGVHD) was discovered to be a risk factor for the occurrence of HCMV viremia (P = .006). The cumulative incidence of HCMV viremia and increasing DNA loads were significantly associated with aGVHD occurrence (P = .001 for each). The occurrence of late-term HCMV viremia was associated with aGVHD (P = .001) and a higher DNA load during the first 12 weeks after HSCT (P = .04). CONCLUSIONS:aGVHD is a risk factor for HCMV viremia. Recipients with aGVHD who have a high HCMV DNA load should be strictly monitored to prevent HCMV activation.

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影响因子:4.51
发表时间:2020-01-01
DOI:10.1016/j.cmi.2019.05.010
作者列表:["Charpentier E","Benichou E","Pagès A","Chauvin P","Fillaux J","Valentin A","Guegan H","Guemas E","Salabert AS","Armengol C","Menard S","Cassaing S","Berry A","Iriart X"]

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分子诊断技术方向

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