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Molecular Profiling of Noncoding Mutations Distinguishes Nevoid Melanomas From Mitotically Active Nevi in Pregnancy.

非编码突变的分子谱分析将妊娠中的痣样黑素瘤与有丝分裂活性痣区分开来。

  • 影响因子:6.06
  • DOI:10.1097/PAS.0000000000001406
  • 作者列表:"Jackett LA","Colebatch AJ","Rawson RV","Ferguson PM","Thompson JF","McCarthy SW","Wilmott JS","Scolyer RA
  • 发表时间:2020-03-01
Abstract

:The accurate recognition of subtle melanomas and their distinction from benign mimics is an oft-recurring diagnostic problem, critical for patient management. Melanomas that bear resemblance to benign nevi (so-called nevoid melanomas, NMs) and benign mitotically active nevi in pregnancy (MANP) are 2 lesions particularly prone to error. Molecular data, including analysis of noncoding regions, in MANP and NM are very limited. This study sought to identify differences in clinical, pathologic, and molecular characteristics between MANP and NMs to facilitate correct diagnosis and reduce the risk of overtreatment or undertreatment. Clinicopathologic characteristics of NM (n=18) and MANP (n=30) were evaluated, and mutation data were analyzed using next-generation sequencing for available cases in each group (NM, n=8; MANP, n=12). All MANP showed innocent histopathologic characteristics apart from increased mitotic activity, frequently in both superficial and deep parts of the lesion (median dermal mitotic rate: 2/mm, range: 1 to 7/mm). All cases of NM demonstrated a characteristic nevoid silhouette, subtle atypical architectural and cytologic features, and variable mitoses (median mitotic rate: 3/mm, range: 1 to 5/mm). Median NM tumor thickness was 1.4 mm. Four of 10 NM patients with follow-up had metastatic disease, including 3 patients who developed widespread metastases, with 1 disease-related death. No other recurrences have been identified (follow-up period: 24 to 60 mo). None of the 15 MANP patients with available follow-up had a recurrence. Most NMs harbored hotspot mutations in NRAS (6/8, 75%). Noncoding mutations were significantly more common in NMs than in MANP (median: 4 vs. 0, P=0.0014). Copy number alterations were infrequent but, when present, were seen in NMs (3/8 NMs vs. 0/12 MANP). All NMs but only 1 of 12 MANP had >1 abnormality in the noncoding regions. Similar to conventional common acquired nevi, MANP mostly harbored driver BRAF mutations, while activating NRAS mutations, noncoding mutations, and copy number alterations were rare. NM and MANP have subtle but recognizable distinguishing histopathologic characteristics that are underpinned by molecular differences. Mutation analysis of targeted noncoding mutations may assist in the diagnosis of difficult lesions.

摘要

: 精确识别微妙的黑色素瘤及其与良性模拟的区别是一个经常重复出现的诊断问题,对患者管理至关重要。与良性痣 (所谓的痣性黑色素瘤,NMs) 和良性妊娠有丝分裂活跃痣 (MANP) 相似的黑色素瘤是2种特别容易出错的病变。在MANP和NM中的分子数据,包括非编码区的分析是非常有限的。本研究旨在确定MANP和NMs之间的临床,病理和分子特征差异,以促进正确诊断并降低过度治疗或治疗不足的风险。评估NM (n = 18) 和MANP (n = 30) 的临床病理特征,并使用下一代测序分析每组中可用病例的突变数据 (NM,n = 8; MANP,n = 12)。除了增加的有丝分裂活动外,所有MANP都显示出无辜的组织病理学特征,通常在病变的浅表和深部 (中值真皮有丝分裂率: 2/mm,范围: 1至7/mm)。NM的所有病例都表现出特征性的痣状轮廓、细微的非典型结构和细胞学特征以及可变的有丝分裂 (中位有丝分裂率: 3/mm,范围: 1至5/mm)。中位NM肿瘤厚度为1.4  mm。随访的10名NM患者中有4名患有转移性疾病,包括3名发生广泛转移的患者,1名疾病相关死亡。未发现其他复发 (随访期: 24 ~ 60 mo mo)。可获得随访的15例MANP患者均未复发。大多数NMs在NRAS中存在热点突变 (6/8,75%)。非编码突变在NMs中比在MANP中显著更常见 (中位数: 4对0,P = 0.0014)。拷贝数改变不常见,但当存在时,在NMs中可见 (3/8 NMs对0/12 MANP)。所有NMs,但12个MANP中只有1个在非编码区有> 1个异常。与传统常见的获得性痣相似,MANP大多具有驱动BRAF突变,而激活NRAS突变、非编码突变和拷贝数改变则罕见。NM和MANP具有微妙但可识别的由分子差异支持的组织病理学特征。靶向非编码突变的突变分析可能有助于疑难病变的诊断。

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影响因子:2.93
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影响因子:0.96
发表时间:2020-01-01
DOI:10.1097/DAD.0000000000001459
作者列表:["Lang UE","Love NR","Cheung C","McCalmont TH","Kim J"]

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皮肤肿瘤方向

皮肤肿瘤是发生在皮肤的细胞增生性疾病,是一种常见病。发生于皮内或皮下组织的新生物,种类很多,临床上分良性肿瘤和恶性肿瘤。恶性肿瘤可以不断增殖,引起转移,威胁生命,称为皮肤癌。

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