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How to Fluorescently Label the Potassium Channel: A Case in hERG.

如何荧光标记钾通道: hERG的一例。

  • 影响因子:3.29
  • DOI:10.2174/0929867326666181129094455
  • 作者列表:"Zhang X","Wang B","Liu Z","Zhou Y","Du L
  • 发表时间:2020-01-01
Abstract

:hERG (Human ether-a-go-go-related gene) potassium channel, which plays an essential role in cardiac action potential repolarization, is responsible for inherited and druginduced long QT syndrome. Recently, the Cryo-EM structure capturing the open conformation of hERG channel was determined, thus pushing the study on hERG channel at 3.8 Å resolution. This report focuses primarily on summarizing the design rationale and application of several fluorescent probes that target hERG channels, which enables dynamic and real-time monitoring of potassium pore channel affinity to further advance the understanding of the channels.

摘要

: hERG (人类乙醚-a-go-相关基因) 钾通道在心脏动作电位复极化中起重要作用,是遗传和药物诱发的长QT综合征的原因。最近,捕获hERG通道开放构象的冷冻-EM结构被确定,从而推动了对hERG通道的研究在3.8 Å 分辨率。本报告主要总结了几种靶向hERG通道的荧光探针的设计原理和应用,这使得能够动态和实时监测钾孔通道亲和力,以进一步推进对通道的理解。

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影响因子:1.41
发表时间:2020-03-01
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作者列表:["Kim S","Namba J","Goodman AM","Nguyen T","Saunders IM"]

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翻译标题与摘要 下载文献
影响因子:1.64
发表时间:2020-01-01
DOI:10.1016/j.hlc.2019.05.170
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翻译标题与摘要 下载文献
心律失常方向

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