小狗阅读会员会员
有解析的医学SCI阅读工具

扫码登录小狗阅读

阅读SCI医学文献

Knockdown of long non-coding RNA PVT1 protects human AC16 cardiomyocytes from hypoxia/reoxygenation-induced apoptosis and autophagy by regulating miR-186/Beclin-1 axis.

敲除长链非编码RNA PVT1通过调节miR-186/Beclin-1轴保护人AC16心肌细胞免受缺氧/复氧诱导的细胞凋亡和自噬。

  • 影响因子:2.60
  • DOI:10.1016/j.gene.2020.144775
  • 作者列表:"Ouyang M","Lu J","Ding Q","Qin T","Peng C","Guo Q
  • 发表时间:2020-09-05
Abstract

:Myocardial ischemia/reperfusion (I/R) injury is a common consequence of restored blood supply after acute myocardial infarction (AMI), but its underlying mechanisms remain largely elusive. In this study, we aimed to investigate the functional role of long non-coding RNA PVT1 in hypoxia/reoxygenation (H/R)-treated AC16 cardiomyocytes. Our experimental results demonstrated that H/R treatment impaired the viability and increased the apoptosis of AC16 cells, and knockdown of PVT1 blocked the H/R injury. Besides, PVT1 knockdown also reduced excessive autophagy in H/R-treated AC16 cells. Furthermore, we confirmed that PVT1 might serve as a ceRNA for miR-186 in AC16 cells, and rescue experiments showed that miR-186 inhibition blocked the effects of PVT1 knockdown in H/R-treated AC16 cells. In summary, this study implied that PVT1 might be a promising therapeutic target for treating myocardial I/R injury.

摘要

: 心肌缺血/再灌注 (I/R) 损伤是急性心肌梗死 (AMI) 后恢复血液供应的常见后果,但其基本机制仍很难捉摸。在本研究中,我们旨在研究长链非编码RNA PVT1在缺氧/复氧 (H/R) 处理的AC16心肌细胞中的功能作用。我们的实验结果表明,H/R处理损害了AC16细胞的活力并增加了细胞凋亡,并且PVT1的敲除阻断了H/R损伤。此外,PVT1敲除还减少了H/R处理的AC16细胞中的过度自噬。此外,我们证实PVT1可能在AC16细胞中作为miR-186的ceRNA,拯救实验显示miR-186抑制阻断了H/R处理的AC16细胞中PVT1敲除的作用。总之,本研究提示PVT1可能是治疗心肌I/R损伤的一个有前景的治疗靶点。

下载该文献
小狗阅读

帮助医生、学生、科研工作者解决SCI文献找不到、看不懂、阅读效率低的问题。提供领域精准的SCI文献,通过多角度解析提高文献阅读效率,从而使用户获得有价值研究思路。

相关文献
影响因子:1.52
发表时间:2020-03-01
DOI:10.1016/j.jtcvs.2019.03.071
作者列表:["Cui H","Schaff HV","Abel MD","Helder MRK","Frye RL","Ommen SR","Nishimura RA"]

METHODS:OBJECTIVE:There has been debate on the importance and pathophysiologic effects of the dynamic subaortic pressure gradient in hypertrophic obstructive cardiomyopathy. The study was conducted to elucidate the hemodynamic abnormalities associated with the dynamic pressure gradient in hypertrophic obstructive cardiomyopathy. METHODS:Eight patients with hypertrophic obstructive cardiomyopathy and 7 patients with valvular aortic stenosis underwent a detailed hemodynamic study of pressure flow relationships before and after myectomy or aortic valve replacement during operation. RESULTS:In aortic stenosis, the increased gradient after premature ventricular contraction was associated with an increase in peak flow (325 ± 122 mL/s to 428 ± 147 mL/s, P = .002) and stroke volume (75.0 ± 27.3 mL to 88.0 ± 24.0 mL, P = .004), but in hypertrophic obstructive cardiomyopathy peak flow remained unchanged (289 ± 79 mL/s to 299 ± 85 mL/s, P = .334) and stroke volume decreased (45.9 ± 18.7 mL to 38.4 ± 14.4 mL, P = .04) on the postpremature ventricular contraction beat. After myectomy, the capacity to augment stroke volume on the postpremature ventricular contraction beats was restored in patients with hypertrophic obstructive cardiomyopathy (45.6 ± 14.4 mL to 54.4 ± 11.8 mL, P = .002). CONCLUSIONS:The pressure flow relationship in hypertrophic obstructive cardiomyopathy supports the concept of true obstruction to outflow, with a low but continued flow during late systole, when the ventricular-aortic pressure gradient is the highest. Septal myectomy can abolish obstruction and restore the ability to augment stroke volume, which may explain the mechanism of symptomatic improvement after operation.

翻译标题与摘要 下载文献
影响因子:1.41
发表时间:2020-03-01
DOI:10.1177/1078155219852146
作者列表:["Gülsaran SK","Baysal M","Demirci U","Baş V","Kirkizlar HO","Umit E","Demir AM"]

METHODS:INTRODUCTION:Ibrutinib, an oral inhibitor of Bruton's tyrosine kinase, has altered the treatment perspective of chronic lymphocytic leukemia and showed modest activity against several types of non-Hodgkin's lymphomas. According to phase studies and real-world data, reported serious adverse effects included atrial fibrillation, diarrhea, and bleeding diathesis. However, heart failure was not reported to be a probable adverse effect linked with ibrutinib. CASE REPORT:In this paper, we present a 66-year-old female chronic lymphocytic leukemia patient who developed significant and symptomatic left ventricular dysfunction at the 13th month of ibrutinib treatment. MANAGEMENT AND OUTCOME:Following cessation of ibrutinib, ejection fraction and clinical findings of the left ventricular dysfunction alleviated. DISCUSSION:Although the use of ibrutinib is generally well tolerated, cardiac functions should be monitored occasionally in all patients.

翻译标题与摘要 下载文献
影响因子:1.21
发表时间:2020-01-01
DOI:10.1007/s11748-019-01164-w
作者列表:["Ishigaki T","Shingu Y","Katoh N","Wakasa S","Katoh H","Ooka T","Kubota S","Matsui Y"]

METHODS:OBJECTIVES:The slope in the preload recruitable stroke work relationship is a highly linear, load-insensitive contractile parameter. However, the perioperative change of the slope has not been reported before. We examined the perioperative slope from a steady-state single beat in patients with functional mitral regurgitation and assessed the correlation with brain natriuretic peptide (BNP) levels. METHODS:The study included 16 patients with non-ischemic dilated cardiomyopathy and refractory heart failure: 10 patients underwent mitral valve plasty and left ventricular plasty (MVP + LVP group) and 6 patients who underwent mitral valve replacement and papillary muscle tugging approximation (MVR + PMTA group). The left ventricular ejection fraction was assessed by the modified Simpson method; the slope was assessed by the single-beat technique using transthoracic echocardiography. BNP levels were measured by chemiluminescent immunoassay. RESULTS:The left ventricular ejection fraction and slope did not significantly change from pre- to early post-surgery in the MVP + LVP group. Both the left ventricular ejection fraction and slope significantly increased 6 months after surgery in the MVR + PMTA group. Postoperative BNP level was low in the MVR + PMTA group. While the postoperative left ventricular ejection fraction did not correlate with BNP levels, the postoperative slope significantly correlated with BNP level after surgery in the MVP + LVP group and in the total functional mitral regurgitation group. CONCLUSIONS:The change of slope was dependent on surgical procedures. In functional mitral regurgitation, the slope may be a more sensitive parameter in reflecting the left ventricular contractile function than the left ventricular ejection fraction.

翻译标题与摘要 下载文献
心肌病方向

心肌病是一组异质性心肌疾病,由不同病因引起心脏机械和电活动的异常,表现为心室不适当的肥厚或扩张。严重心肌病会引起心血管性死亡或进展性心力衰竭。心肌病通常分为原发性心肌病和继发性心肌病,其中原发性心肌病包括扩张型心肌病、肥厚型心肌病、限制型心肌病、致心律失常性右室心肌病和未定型心肌病。继发性心肌病指心肌病是全身性疾病的一部分。

复制标题
发送后即可在该邮箱或我的下载查看该文献
发送
该文献默认存储到我的下载

科研福利

报名咨询

建议反馈
问题标题:
联系方式:
电子邮件:
您的需求: