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circPUM1 promotes polycystic ovary syndrome progression by sponging to miR-760.

circPUM1通过海绵至miR-760促进多囊卵巢综合征进展。

  • 影响因子:2.60
  • DOI:10.1016/j.gene.2020.144903
  • 作者列表:"Deng L","Chen Q","Xie J","Wei W","Hui H
  • 发表时间:2020-09-05
Abstract

:Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among reproductive-age women. The circRNA-miRNA axis functions in various diseases progression have been partially revealed in the past two decades. However, little is known about the role of the circRNA-miRNA axis in PCOS progression. MicroRNA miR-760, which is characterized by tissue-specific, has been studied in several cancers. Firstly, we found that miR-760 expression was decreased in PCOS tissues insulin treated GCs, KGN and SVOG cells. Secondly, The CCK-8 and apoptosis experiment results suggested that downregulated miR-760 promoted cell proliferation ability and suppressed apoptosis activity in KGN and SVOG cells. Then, the bioinformatic analysis result indicated that circPUM1 was a potential sponge to miR-760. By performing AGO2-RIP, RNA pull-down, Luciferase reporter, and qRT-PCR experiments, we demonstrated that circPUM1 acted as a molecular sponge to miR-760, and decreased miR-760 expression. Moreover, it was found that the promotive effect of circPUM1 was mediated by regulating miR-760. Collectively, our findings suggest that circPUM1 promotes PCOS progression through sponging to miR-760. We may provide a promising therapeutic target for PCOS.

摘要

多囊卵巢综合征 (PCOS) 是育龄妇女最常见的内分泌疾病之一。在过去的二十年中,已经部分揭示了circRNA-miRNA轴在各种疾病进展中的功能。然而,关于circRNA-miRNA轴在PCOS进展中的作用知之甚少。已经在几种癌症中研究了以组织特异性为特征的微小rna miR-760。首先,我们发现胰岛素处理的GCs、KGN和SVOG细胞中PCOS组织miR-760表达降低。其次,CCK-8和凋亡实验结果提示,下调miR-760促进KGN和SVOG细胞的增殖能力,抑制凋亡活性。然后,生物信息学分析结果表明circPUM1是潜在的海绵至miR-760。通过进行AGO2-RIP、RNA下拉、荧光素酶报告和qRT-PCR实验,我们证明了circPUM1作为分子海绵起到miR-760的作用,并降低了miR-760的表达。此外,发现circPUM1的促进作用是通过调节miR-760介导的。总的来说,我们的研究结果表明circPUM1通过海绵miR-760促进PCOS进展。我们可能为PCOS提供一个有前景的治疗靶点。

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影响因子:2.42
发表时间:2020-02-01
DOI:10.1007/s12011-019-01715-5
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影响因子:2.42
发表时间:2020-02-01
DOI:10.1007/s12011-019-01720-8
作者列表:["Jamilian M","Foroozanfard F","Kavossian E","Kia M","Aghadavod E","Amirani E","Asemi Z"]

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囊肿方向

囊肿是一种良性疾病,它可以长在人体表面,也可以长在内脏里;囊肿就是长在体内某一脏器、囊状的良性包块,其内容物的性质是液态的。

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