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SEN virus genotype H distribution in β-thalassemic patients and in healthy donors in Iraq: Molecular and physiological study.

伊拉克 β-地中海贫血患者和健康供者的SEN病毒基因型H分布: 分子和生理学研究。

  • 影响因子:4.33
  • DOI:10.1371/journal.pntd.0007880
  • 作者列表:"Al-Ouqaili MTS","Majeed YH","Al-Ani SK
  • 发表时间:2020-06-08
Abstract

:The SEN virus (SENV) has been linked to transfusion-associated non-A-E hepatitis; however, information regarding SENV infections in patients with thalassemia, particularly in those with hepatitis virus co-infections, remains limited. This study investigated the frequency of SENV (genotypes D and H) infections in Iraqi patients with thalassemic patients infected and not infected with hepatitis C virus. The study involved 150 β-thalassemia patients (75 with HCV infections and 75 without) and 75 healthy blood donors. Patient levels of vitamins C and E, liver function markers, and glutathione peroxidase (GPX) were determined. Recovered viral nucleic acids were amplified using the conventional polymerase chain reaction (SENV DNA) or the real-time polymerase chain reaction (HCV RNA) techniques. Only 10% of healthy donors had evidence of SENV infection. Among patients with thalassemia, 80% and 77% of patients with and without concurrent HCV infections, respectively, had SENV infections. DNA sequencing analyses were performed on blood samples obtained from 29 patients. Patients with thalassemia, particularly those with SENV infections, had higher levels of several enzymatic liver function markers and total serum bilirubin (P < 0.05) than did healthy blood donors. Among the examined liver function markers, only gamma-glutamyl transferase demonstrated significantly higher levels in HCV-negative patients infected with SENV-H than in those infected with SENV-D (P = 0.01). There were significantly lower vitamin C, vitamin E, and glutathione peroxidase levels in patients than in healthy donors (P < 0.05), but only glutathione peroxidase levels were significantly lower in HCV-negative thalassemia patients infected with SENV than in those without SENV infections (P = 0.04). The SENV-H genotype sequences were similar to the global standard genes in GenBank. These results broaden our understanding the nature of the SENV-H genotype and the differential role of SENV-H infections, compared to SENV-D infections, in patients with thalassemia, in Iraq.

摘要

: SEN病毒 (SENV) 与输血相关的非A-E型肝炎有关; 然而,关于地中海贫血患者,特别是合并肝炎病毒感染者的SENV感染的信息仍然有限。本研究调查了伊拉克地中海贫血患者感染和未感染丙型肝炎病毒的SENV (基因型D和H) 感染频率。该研究涉及150例 β-地中海贫血患者 (75例HCV感染和75例无) 和75例健康献血者。测定患者的维生素c和E、肝功能标志物和谷胱甘肽过氧化物酶 (GPX) 水平。使用常规聚合酶链反应 (senvdna) 或实时聚合酶链反应 (hcvrna) 技术扩增回收的病毒核酸。只有10% 的健康供体有SENV感染的证据。在地中海贫血患者中,合并和未合并HCV感染的患者分别有80% 和77% 的SENV感染。对从29名患者获得的血液样品进行DNA测序分析。与健康献血者相比,地中海贫血患者,尤其是SENV感染患者的几种酶促肝功能标志物和总血清胆红素水平更高 (P <0.05)。在检查的肝功能标志物中,只有 γ-谷氨酰转移酶在感染senv-h的HCV阴性患者中表现出显著高于感染senv-d的患者 (P = 0.01)。患者的维生素c、维生素e和谷胱甘肽过氧化物酶水平显著低于健康供者 (P <0.05),但感染SENV的HCV阴性地中海贫血患者的谷胱甘肽过氧化物酶水平显著低于未感染SENV的患者 (P = 0.04)。SENV-H基因型序列与GenBank中的全球标准基因相似。这些结果拓宽了我们对伊拉克地中海贫血患者中SENV-H基因型的性质和SENV-H感染与SENV-D感染相比的差异作用的理解。

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影响因子:2.36
发表时间:2020-01-01
来源期刊:Platelets
DOI:10.1080/09537104.2019.1581922
作者列表:["Szanto T","Nummi V","Jouppila A","Brinkman HJM","Lassila R"]

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主要包括小儿贫血、血友病、急性白血病及特发性血小板减少性紫癫等疾病。

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