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The effects of barium concentration levels on the pulmonary inflammatory response in a rat model of aspiration.

钡剂浓度水平对大鼠误吸模型肺部炎症反应的影响。

  • 影响因子:1.65
  • DOI:10.1007/s00405-019-05666-4
  • 作者列表:"Ueha R","Nativ-Zeltzer N","Sato T","Goto T","Yamauchi A","Belafsky PC","Yamasoba T
  • 发表时间:2020-01-01
Abstract

PURPOSE:Barium sulfate (Ba) suspension is the most widely used contrast agent for upper gastrointestinal and videofluoroscopic swallow studies (VFSS). The effect of Ba concentration on lung injury is uncertain. The aims of this study were to explore the effects of different barium concentrations on the respiratory organs and elucidate the underlying mechanisms of these effects in an established animal model of aspiration. METHODS:Animal model study. Eight-week-old male Sprague-Dawley rats were allocated into three groups (n = 12, each group). Two groups underwent tracheal instillation of low (30% w/v) and high (60% w/v) concentration Ba (low-Ba, high-Ba). A control group was instilled with saline. Half of the animals were euthanized on day 2 and the remaining half were euthanized on day 30. Histological and gene analyses were performed. RESULTS:Both low-Ba and high-Ba aspiration caused inflammatory cell infiltration in the lung at 2 days post aspiration with an increase in the expression of inflammatory cytokines. At 30 days post aspiration, small quantities of barium particles remained in the lung of the low-Ba group without any inflammatory reaction. Chronic inflammation was recognized in the high-Ba group up to 30 days post aspiration. CONCLUSION:A small amount of high concentration Ba (60% w/v) caused sustained inflammation in the rat lung at least 30 days after aspiration. Even with a small amount of low concentration Ba aspiration (30% w/v), Ba particles can remain in the lung over a month, causing sustained late effects.

摘要

目的: 硫酸钡 (Ba) 混悬液是上消化道和视频透视吞咽研究 (VFSS) 中应用最广泛的造影剂。Ba 浓度对肺损伤的影响还没有定论。本研究旨在探讨不同钡剂浓度对呼吸器官的影响,并在已建立的误吸动物模型中阐明这些影响的潜在机制。 方法: 动物模型研究。8 周龄雄性 Sprague-Dawley 大鼠分为三组 (n = 12,每组)。两组均行低 (30% w/v) 、高 (60% w/v) 浓度 Ba (低-Ba 、高-Ba) 气管滴注。对照组滴注生理盐水。一半的动物在第 2 天安乐死,其余一半在第 30 天安乐死。进行组织学和基因分析。 结果: 低 Ba 和高 Ba 吸入均可引起肺内炎性细胞浸润,且炎性细胞因子表达增加。抽吸后 30 天,低 Ba 组肺内仍有少量钡颗粒,无任何炎症反应。高 Ba 组在误吸后 30 天内发现慢性炎症。 结论: 少量高浓度 Ba (60% w/v) 在吸入后至少 30d 引起大鼠肺部持续炎症。即使少量低浓度 Ba 吸入 (30% w/v),Ba 颗粒仍可在肺中停留一个月,引起持续的晚期效应。

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影响因子:3.94
发表时间:2020-01-15
DOI:10.1016/j.taap.2019.114847
作者列表:["Bernstein DM","Toth B","Rogers RA","Kling DE","Kunzendorf P","Phillips JI","Ernst H"]

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影响因子:4.04
发表时间:2020-01-10
DOI:10.1042/BST20191010
作者列表:["Zaragosi LE","Deprez M","Barbry P"]

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