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Localisation of cannabinoid and cannabinoid-related receptors in the equine dorsal root ganglia.

大麻素和大麻素相关受体在马背根神经节中的定位。

  • 影响因子:1.86
  • DOI:10.1111/evj.13305
  • 作者列表:"Chiocchetti R","Rinnovati R","Tagliavia C","Stanzani A","Galiazzo G","Giancola F","Silva M","Capodanno Y","Spadari A
  • 发表时间:2021-05-01
Abstract

BACKGROUND:Growing evidence recognises cannabinoid receptors as potential therapeutic targets for pain. Consequently, there is increasing interest in developing cannabinoid receptor agonists for treating pain. As a general rule, to better understand the actions of a drug, it would be of extreme importance to know the cellular distribution of its specific receptors. The localisation of cannabinoid receptors in the dorsal root ganglia of the horse has not yet been investigated. OBJECTIVES:To localise the cellular distribution of canonical and putative cannabinoid receptors in the equine cervical dorsal root ganglia. STUDY DESIGN:Qualitative and quantitative immunohistochemical study. METHODS:Cervical (C6-C8) dorsal root ganglia were collected from six horses (1.5 years of age) at the slaughterhouse. The tissues were fixed and processed to obtain cryosections which were used to investigate the immunoreactivity of canonical cannabinoid receptors 1 (CB1R) and 2 (CB2R), and for three putative cannabinoid-related receptors: nuclear peroxisome proliferator-activated receptor alpha (PPARα), transient receptor potential ankyrin 1 (TRPA1) and serotonin 5-HT1a receptor (5-HT1aR). RESULTS:The neurons showed immunoreactivity for CB1R (100%), CB2R (80% ± 13%), PPARα (100%), TRPA1 (74% ± 10%) and 5-HT1aR (84% ± 6%). The neuronal satellite glial cells showed immunoreactivity for CB2R, PPARα, TRPA1 and 5-HT1aR. MAIN LIMITATIONS:The low number of horses included in the study. CONCLUSIONS:This study highlighted the expression of cannabinoid receptors in the sensory neurons and glial cells of the dorsal root ganglia. These findings could be of particular relevance for future functional studies assessing the effects of cannabinoids in horses to manage pain.

摘要

背景: 越来越多的证据认为大麻素受体是疼痛的潜在治疗靶点。因此,开发用于治疗疼痛的大麻素受体激动剂的兴趣日益增加。作为一般规则,为了更好地理解药物的作用,了解其特异性受体的细胞分布将是极其重要的。大麻素受体在马的背根神经节中的定位尚未被研究。 目的: 定位规范和推定的大麻素受体在马颈背根神经节中的细胞分布。 研究设计: 定性和定量免疫组化研究。 方法: 在屠宰场收集6匹马 (1.5岁) 的颈 (C6-C8) 背根神经节。组织被固定和加工以获得冷冻切片,用于研究典型大麻素受体1 (CB1R) 和2 (CB2R) 的免疫反应性,以及三种推定的大麻素相关受体: 核过氧化物酶体增殖物激活受体 α (ppar α),瞬时受体电位锚蛋白1 (TRPA1) 和5-羟色胺5-HT1a受体 (5-HT1aR)。 结果: 神经元呈CB1R (100%) 、CB2R (80% ± 13%) 、ppar α (100%) 、TRPA1 (74% ± 10%) 和5-HT1aR (84% ± 6%) 免疫反应性。神经元卫星胶质细胞对CB2R、ppar α 、TRPA1和5-HT1aR显示免疫反应性。 主要限制: 研究中包括的马的数量少。 结论: 本研究强调了大麻素受体在背根神经节的感觉神经元和神经胶质细胞中的表达。这些发现可能与未来评估大麻素在马中控制疼痛的作用的功能研究特别相关。

关键词: 5-HT1aR CB1 CB2 Ppar α TRPA1
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影响因子:2.68
发表时间:2021-02-01
DOI:10.1080/14656566.2020.1814255
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DOI:10.1007/s11033-021-06299-9
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