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Parathyroid hormone receptor stimulation induces human adipocyte lipolysis and browning.
甲状旁腺激素受体刺激诱导人脂肪细胞脂肪分解和褐变。
- 影响因子:0
- DOI:10.1530/EJE-20-0713
- 作者列表:"Breining P","Pedersen SB","Kjolby M","Hansen JB","Jessen N","Richelsen B
- 发表时间:2021-05-01
Abstract
Objective:Activation of brown adipose tissue is a promising strategy to treat and prevent obesity and obesity-related disorders. Activation of uncoupling protein 1 (UCP1) leads to uncoupled respiration and dissipation of stored energy as heat. Induction of UCP1-rich adipocytes in white adipose tissue, a process known as 'browning', serves as an alternative strategy to increase whole body uncoupling capacity. Here, we aim to assess the association between parathyroid hormone (PTH) receptor expression and UCP1 expression in human adipose tissues and to study PTH effects on human white and brown adipocyte lipolysis and UCP1 expression. Design:A descriptive study of human neck adipose tissue biopsies substantiated by an interventional study on human neck-derived adipose tissue cell models. Methods:Thermogenic markers and PTH receptor gene expression are assessed in human neck adipose tissue biopsies and are related to individual health records. PTH-initiated lipolysis and thermogenic gene induction are assessed in cultured human white and brown adipocyte cell models. PTH receptor involvement is investigated by PTH receptor silencing. Results:PTH receptor gene expression correlates with UCP1 gene expression in the deep-neck adipose tissue in humans. In cell models, PTH receptor stimulation increases lipolysis and stimulates gene transcription of multiple thermogenic markers. Silencing of the PTH receptor attenuates the effects of PTH indicating a direct PTH effect via this receptor. Conclusion:PTH 1 receptor stimulation by PTH may play a role in human adipose tissue metabolism by affecting lipolysis and thermogenic capacity.
摘要
目的: 激活棕色脂肪组织是治疗和预防肥胖和肥胖相关疾病的有前途的策略。解偶联蛋白1 (UCP1) 的激活导致解偶联呼吸和作为热的储存能量的耗散。白色脂肪组织中UCP1-rich脂肪细胞的诱导 (称为 “褐色” 的过程) 作为增加全身解偶联能力的替代策略。在这里,我们的目的是评估人类脂肪组织中甲状旁腺激素 (PTH) 受体表达和UCP1表达之间的关联,并研究PTH对人白色和棕色脂肪细胞脂肪分解和UCP1表达的影响。 设计: 人类颈部脂肪组织活检的描述性研究,由人类颈部脂肪组织细胞模型的干预性研究证实。 方法: 在人颈部脂肪组织活检中评估产热标记物和PTH受体基因表达,并与个体健康记录相关。在培养的人白色和棕色脂肪细胞模型中评估PTH引发的脂肪分解和产热基因诱导。通过PTH受体沉默研究PTH受体参与。 结果: 人类深颈脂肪组织中PTH受体基因表达与UCP1基因表达相关。在细胞模型中,PTH受体刺激增加脂肪分解并刺激多个产热标记物的基因转录。PTH受体的沉默减弱了PTH的作用,表明通过该受体的直接PTH作用。 结论: PTH刺激PTH 1受体可能通过影响脂肪分解和产热能力而在人体脂肪组织代谢中发挥作用。
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