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A nanotechnology-based new approach in the treatment of breast cancer: Biosynthesized silver nanoparticles using Cuminum cyminum L. seed extract.

基于纳米技术的乳腺癌治疗新方法: 使用 cyminum L. seed extract 生物合成银纳米颗粒。

  • 影响因子:3.92
  • DOI:10.1016/j.jphotobiol.2020.111902
  • 作者列表:"Dinparvar S","Bagirova M","Allahverdiyev AM","Abamor ES","Safarov T","Aydogdu M","Aktas D
  • 发表时间:2020-07-01
Abstract

:The present study reports the anticancer activities of Cuminum cyminum L. (Cumin) seed extract, chemically synthetized silver nanoparticles (AgNPs) and biosynthesized silver nanoparticles (Bio-AgNPs) from Cumin seeds on human breast adenocarcinoma cell line (MCF-7) and human breast adenocarcinoma metastatic cell line (AU565). The synthetized nanoparticles were characterized by dynamic light scattering (DLS), UV-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The cytotoxic and anticancer effects of AgNPs and Bio-AgNPs were determined by MTT assay. According to the cytotoxicity analysis, Bio-AgNPs appears to be less toxic against J774 macrophage cells than AgNPs since IC50 values were measured as 0.75 and 1.25 μg/ml for AgNPs and Bio-AgNPs, respectively. On the other hand, Bio-AgNPs demonstrated significant inhibitory effects on human breast cancer cells at non-toxic concentrations such as 0.25 and 0.5 μg/ml. However, at increased concentrations, the lethal effects of AgNPs on breast cancer cells were higher than Bio-AgNPs. When cytotoxic and anticancer characteristics of Cumin extract were investigated, it was established that it did not show any inhibitory effect on J774 cells, while killing the half of MCF-7 cells at investigated concentrations. Interestingly, Cumin extract gave rise to no inhibitory effects against AU565 cells. On the other hand, AgNPs and Bio-AgNPs exhibited considerable anticancer activities on both cell lines. The inhibition percentages of AgNPs on MCF-7 and AU565 cell lines were respectively evaluated as 95% and 97% at the highest concentrations applied (12.5 μg/ml). Similarly, we determined that 87.5% and 96% of MCF-7 and AU565 cells were respectively inhibited when they were exposed to the highest concentrations of Bio-AgNPs. Considering relatively toxic-free features of Bio-AgNPs prepared from Cuminum cyminum L. seed extracts, it can be thought that this formulation will be a pioneer in development of nanotechnology-based new anticancer drug for the treatment of breast cancer in near future.

摘要

: 本研究报道了 cyminum L.的抗癌活性。(孜然) 种子提取物,化学合成的银纳米颗粒 (AgNPs) 和生物合成的银纳米颗粒 (Bio-AgNPs) 从孜然种子对人乳腺腺癌细胞系 (MCF-7) 和人乳腺腺癌转移细胞系 (AU565)。通过动态光散射 (DLS) 、紫外-可见光谱 (UV-Vis) 、 x射线衍射 (XRD) 、傅里叶变换红外光谱 (FTIR) 对合成的纳米颗粒进行了表征。和扫描电子显微镜 (SEM)。MTT 法测定 AgNPs 和 Bio-AgNPs 的细胞毒性和抗癌作用。根据细胞毒性分析,Bio-AgNPs 对 J774 巨噬细胞的毒性似乎比 AgNPs 小,因为测得 AgNPs 和 Bio-AgNPs 的 IC50 值分别为 0.75 和 1.25 μ g/ml,分别。另一方面,Bio-AgNPs 在无毒浓度如 0.25 和 0.5 μ g/ml 时对人乳腺癌细胞表现出显著的抑制作用。然而,在浓度增加时,AgNPs 对乳腺癌细胞的致死作用高于 Bio-AgNPs。当研究孜然提取物的细胞毒性和抗癌特性时,确定它对 J774 细胞没有表现出任何抑制作用,而在研究浓度下杀死 MCF-7 细胞的一半。有趣的是,孜然提取物对 AU565 细胞没有抑制作用。另一方面,AgNPs 和 Bio-AgNPs 在两种细胞系上都表现出相当大的抗癌活性。在最高浓度 (95% μ g/ml) 时,AgNPs 对 MCF-7 和 AU565 细胞的抑制率分别为 97% 和 12.5。同样,我们确定当它们暴露于最高浓度的 Bio-AgNPs 时,分别有 87.5% 和 96% 的 MCF-7 和 AU565 细胞受到抑制。考虑由 cyminum L.制备的 Bio-AgNPs 的相对无毒性特征。种子提取物,可以认为该制剂将在不久的将来成为开发基于纳米技术的治疗乳腺癌的新型抗癌药物的先驱。

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影响因子:2.87
发表时间:2020-01-31
来源期刊:Bioscience reports
DOI:10.1042/BSR20192546
作者列表:["Chen X","Theobard R","Zhang J","Dai X"]

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DOI:10.1186/s12885-020-6534-z
作者列表:["Soliman H","Shah V","Srkalovic G","Mahtani R","Levine E","Mavromatis B","Srinivasiah J","Kassar M","Gabordi R","Qamar R","Untch S","Kling HM","Treece T","Audeh W"]

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