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Utility of FOS as diagnostic marker for osteoid osteoma and osteoblastoma.

FOS作为骨样骨瘤和成骨细胞瘤诊断标志物的效用。

  • 影响因子:2.52
  • DOI:10.1007/s00428-019-02684-9
  • 作者列表:"Lam SW","Cleven AHG","Kroon HM","Briaire-de Bruijn IH","Szuhai K","Bovée JVMG
  • 发表时间:2020-03-01
Abstract

:Osteoid osteoma and osteoblastoma are bone-forming tumors shown to harbor FOS (87%) and FOSB (3%) rearrangements. The aim was to evaluate the immunohistochemical expression of FOS and FOSB in these tumors in comparison to other bone tumors, to evaluate the influence of decalcification, and to correlate immunohistochemical findings with the underlying genetic alteration using fluorescence in situ hybridization (FISH). Immunohistochemistry using whole sections was performed on osteoid osteoma (n=23), osteoblastoma (n=22), osteoblastoma-like osteosarcoma (n=3), reactive (n=3), and proliferative (n=11) bone lesions. Immunoreactivity in giant cell tumor of bone (n=74), aneurysmal bone cyst (n=6), chondromyxoid fibroma (n=20), osteosarcoma (n=85), chondroblastoma (n=17), and clear cell chondrosarcoma (n=20) was assessed using tissue micro arrays. Strong nuclear expression of FOS in > 50% of the tumor cells was observed in all osteoid osteomas (22/22), in 57% of osteoblastomas (12/21) and in 3/197 control cases. FOS immunoreactivity disappeared after > 3 days decalcification. FOS rearrangements were present in 94% of osteoid osteomas and osteoblastomas, with a concordance of 86% between FISH and immunohistochemistry. Two osteoblastomas (5%) were positive for FOSB, as opposed to 8/177 control cases. Additional FISH revealed no FOSB rearrangements in these cases. To conclude, in short decalcified biopsies, FOS immunohistochemistry can be used to diagnose osteoid osteoma and osteoblastoma, as overexpression is seen in the majority, being rare in their mimics. FOS immunohistochemistry should not be used after long decalcification. Moreover, low level of focal expression found in other lesions and tissues might cause diagnostic problems, in which case FISH could be employed.

摘要

: 骨样骨瘤和骨母细胞瘤是显示有FOS (87%) 和FOSB (3%) 重排的骨形成肿瘤。目的是评估FOS和FOSB在这些肿瘤中的免疫组织化学表达与其他骨肿瘤的比较,评估脱钙的影响,并使用荧光原位杂交 (FISH) 将免疫组化结果与潜在的遗传改变相关联。对骨样骨瘤 (n = 2 3) 、骨母细胞瘤 (n = 22) 、骨母细胞瘤样骨肉瘤 (n = 3) 、反应性 (n = 3) 、和增生性 (n = 11) 骨病变。骨巨细胞瘤 (n = 74),动脉瘤样骨囊肿 (n = 6),软骨黏液样纤维瘤 (n = 20),骨肉瘤 (n = 85),使用组织微阵列评估软骨母细胞瘤 (n = 17) 和透明细胞软骨肉瘤 (n = 20)。较强的核FOS表达> 50% 的肿瘤细胞中观察到所有骨样骨瘤 (22/22),在 57% 的osteoblastomas (12/21) 和 3/197 例对照组.> 3 天脱钙后FOS免疫反应性消失。94% 的骨样骨瘤和成骨细胞瘤存在FOS重排,FISH和免疫组化的一致性为 86%。2 例成骨细胞瘤 (5%) FOSB阳性,而对照组为 8/177 例。在这些情况下,额外的鱼未发现FOSB重排。总之,总之,脱钙活检,FOS免疫组化可用于诊断骨样骨瘤和骨母细胞瘤,因为在大多数情况下可见过表达,在其模拟物中很少见。长期脱钙后不宜使用FOS免疫组化。此外,在其他病变和组织中发现低水平的局灶性表达可能会导致诊断问题,在这种情况下可以使用FISH。

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影响因子:1.41
发表时间:2020-03-01
DOI:10.1177/1078155219842277
作者列表:["Gyori DJ","Bullington SM","Crawford BS","Vernon VP"]

METHODS:BACKGROUND:National guidelines recommend screening and treatment for cancer-related bone disease and continued monitoring of bone-modifying agents. It is unclear whether a standardized screening tool is utilized to identify eligible patients and ensure appropriate supportive care is implemented. The purpose of this study was to evaluate current prescribing practices and optimize management of bone-modifying agents. METHODS:A retrospective chart review was performed to identify patients who received hormone deprivation therapy or had bone metastases through Hematology/Oncology or Urology clinics from 1 November 2016 to 31 October 2017. The primary endpoints of this study were the incidence of completed baseline dual-energy X-ray absorptiometry (DEXA) scan for patients on hormone deprivation therapy and percent of patients started on a bone-modifying agent for the prevention of skeletal-related events secondary to bone metastasis. Secondary endpoints included percent of patients with dental examinations prior to initiation, adequate calcium and vitamin D supplementation, incidence of osteonecrosis of the jaw or flu-like symptoms and education, and percent of bisphosphonate doses appropriately adjusted based on renal function. RESULTS:A total of 375 patients were assessed for baseline DEXA scans and bone-modifying therapy. Of the 226 patients on hormone deprivation therapy, 111 (49%) patients were appropriately screened with a DEXA scan prior to initiation of hormone deprivation therapy. Among the 149 patients with bone metastases, only 94 (63.1%) patients were started on a bone-modifying agent. CONCLUSIONS:Opportunities have been identified to optimize management of patients with cancer-related bone disease. Implementation of standardized tools may increase the rate of appropriate screening and initiation of bone-modifying therapy when warranted.

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影响因子:2.83
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DOI:10.1007/s00520-019-04843-9
作者列表:["Dohzono S","Sasaoka R","Takamatsu K","Hoshino M","Nakamura H"]

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骨肿瘤方向

骨肿瘤是发生于骨骼或其附属组织的肿瘤。有良性,恶性之分,良性骨肿瘤易根治,预后良好,恶性骨肿瘤发展迅速,预后不佳,死亡率高。

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