Carbon dots derived from lychee waste: Application for Fe3+ ions sensing in real water and multicolor cell imaging of skin melanoma cells.
来源于荔枝废物的碳点: 应用Fe3 + 离子传感在真实水和皮肤黑色素瘤细胞的多色细胞成像。
- 作者列表："Sahoo NK","Jana GC","Aktara MN","Das S","Nayim S","Patra A","Bhattacharjee P","Bhadra K","Hossain M
:Exploit of biomass as an inexhaustible resource has accepted much more curiosity to the present research world. Herein, a simple, one-step solvothermal action has been used to synthesize an ascendable amount of fluorescent carbon dots (CDs) with an average size of~3.13 nm, from Low-reasonable and green source lychee waste. The excitation/emission maxima of CDs have 365/443 nm with high quantum yield (23.5%). The present ingredient predominantly contained carboxylic acid and hydroxyl group that acted as a passive agent for stabilizing the CDs. The structural and optical properties were evaluated through HRTEM, FTIR, UV-vis, zeta potential, XPS, fluorescence, and fluorescence lifetime experiments. We investigated the manoeuvre of our synthesized CDs as a probe for detection of Fe3+ ions in water bodies; This sensing approach showed impressive selectivity and sensitivity towards Fe3+ions with LOD 23.6 nM. The sensing mechanism took place through static quenching which was entrenched through fluorescence lifetime measurements. Fe3+ ions detection was basically carried out with efficacy in real water. For its lofty Photo-stability, low cytotoxicity and cell viability the probe were substantially applied for bio-imaging experiment i.e. intracellular multi-color cell imaging in skin melanoma cells (A375 cells) with and without Fe3+ ions exemplifying its real applications in living cells.
: 生物质作为一种取之不尽、用之不竭的资源，对目前的研究世界已经接受了更多的好奇心。在此，使用简单的一步溶剂热作用合成了平均尺寸为 ~ 3.13 nm的可增加数量的荧光碳点 (CDs)，从低-合理和绿色来源荔枝废物。CDs的激发/发射极大值为 365/443 nm，量子产率高 (23.5%)。本成分主要含有羧酸和羟基，作为稳定CDs的被动剂。通过HRTEM、FTIR、UV-vis、zeta电位、XPS、荧光和荧光寿命实验评价了其结构和光学性能。我们研究了我们合成的CDs作为探测水体中Fe3 + 离子的探针的操作; 这种传感方法对LOD为 23.6 nm的Fe3 + 离子表现出令人印象深刻的选择性和灵敏度。传感机制是通过静态猝灭发生的，通过荧光寿命测量根深蒂固。在实际水中基本上进行了Fe3 + 离子的有效检测。由于其高度的光稳定性、低细胞毒性和细胞活力，该探针被大量应用于生物成像实验，即含和不含Fe3 + 离子的皮肤黑色素瘤细胞 (A375 细胞) 的细胞内多色细胞成像举例说明其在活细胞中的真正应用。
METHODS::Blue rubber bleb naevus syndrome (BRBNS) is an extremely rare venous malformation that often manifests as multiple haemangioma-like lesions in the skin and gastrointestinal tract. The drug sirolimus plays a key role in the signalling pathway of angiogenesis and subsequent development of BRBNS and its use has been described in several case reports. We present a case series of four patients with BRBNS who exhibited good treatment response to sirolimus. All four patients were administered oral sirolimus at doses of 1.0-1.5 mg/m2 /day with a target drug level of 5-10 ng/mL and median treatment duration of 20 months. All patients had a reduction in the size of the lesions and a normalization of coagulopathy with tolerable drug adverse reactions at follow-up. Sirolimus may be effective and safe in paediatric patients with BRBNS. Further prospective studies are suggested to evaluate the long-term effectiveness of this drug.
METHODS:BACKGROUND:Human papillomavirus (HPV) infections are associated with common dermatologic and nondermatologic diseases. Although HPV vaccines are well established as preventive measures for genital warts and cervical neoplasia, their use as therapeutic agents deserves greater attention. OBJECTIVE:To evaluate the use of HPV vaccine(s) as a treatment modality for cutaneous and/or mucosal disease. METHODS:A primary literature search using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines was conducted in January 2019 by using the PubMed and Cochrane databases. RESULTS:A total of 63 articles with 4439 patients were included. The majority of patients with cutaneous warts, recurrent respiratory papillomatosis, and squamous and basal cell carcinomas were successfully treated with HPV vaccination. Preliminary data on patients with pre-existing anogenital warts, cervical intraepithelial neoplasia, anal intraepithelial neoplasia, and vulvar intraepithelial neoplasia is promising. LIMITATIONS:This review was limited by the lack of controls, patients' previous HPV vaccination status, and publication bias. CONCLUSION:The commercially available three-dose, quadrivalent HPV vaccine is a potential therapeutic option for the treatment of cutaneous warts, recurrent respiratory papillomatosis, and squamous and basal cell carcinomas. Noncommercially available HPV vaccines demonstrate therapeutic response for treating anogenital warts, cervical intraepithelial neoplasia, anal intraepithelial neoplasia, and vulvar intraepithelial neoplasia. The vaccine's efficacy as an adjunct therapy for HPV-associated cutaneous and/or mucosal disease warrants further exploration.
METHODS::Our understanding of melanoma precursors and progression to melanoma has developed as a result of advances in the field of molecular diagnostics. We now better understand the potential for genetic heterogeneity within a single lesion. Combined tumors can pose a diagnostic challenge when deciding the line between benign and malignant, which in turn has direct implications for patient management. Primary cilia (PC) are ubiquitous sensory organelles that have essential functions in cellular proliferation, differentiation, and development. The ciliation index (percentage of ciliated melanocytes) has been shown to reliably differentiate melanoma, which fail to ciliate, from melanocytic nevi, which retain PC. We therefore analyzed the potential for using the ciliation index to differentiate benign and malignant components in combined melanocytic lesions. We collected patient samples (n = 10) of unequivocal combined lesions with both melanoma and associated nevus components. Melanocytes were highlighted with SOX10 and costained with gamma-Tubulin and acetylated alpha-Tubulin to highlight the basal body and cilium, respectively. The number of melanocytes retaining cilia under high-power microscopy was examined. The melanoma component had average of 4% ciliation (SD: 7%), whereas the associated nevus component was significantly higher with 59% ciliation (SD: 17%). These data show that PC may be a reliable means of distinguishing benign from malignant components within a single tumor. The ciliation index may be a helpful tool in distinguishing challenging cases of combined lesions of melanoma in situ with a dermal nevus component from invasive melanoma, thus promoting improved staging and clinical management.