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[Isolation and Biological Characterization of Lung Cancer Stem Cells].

[肺癌干细胞隔离生物学特性]。

  • 影响因子:0
  • DOI:10.3881/j.issn.1000-503X.10911
  • 作者列表:"Zhang ZF","Gao F","Wang T","Wang R","Guo Y","Zhang GL","Zeng H","Liu CJ
  • 发表时间:2020-02-28
Abstract

:Objective To explore the methods of screening and biological characteristics of lung cancer stem cells. Methods We selected the ABCG2 +and ABCG2 -cells from SPC-A-1/adriamycin(ADM)cell line induced by ADM and analyzed the tumorigenicity of ABCG2 +and ABCG2 -cells in vivo by flow cytometry and transplantation in nude mice. Results The average fluorescence intensity of SPC-A-1 cells was(1.001±0.014)×10 2,which was significantly lower than that of SPC-A-1/ADM cells [(10.257±0.023) ×10 2 ](t=17.320,P=0.001);the difference was also statistically significant between the ABCG2/BCRP-FITC treatment group and the SPC-A-1 control group(t=5.269,P=0.021) and the SPC-A-1 control group(t=6.869, P=0.012) and between the SPC-A-1/ADM cell control group and the SPC-A-1/ADM cell homotype control group(t=8.112,P=0.015).The positive rate of SPC-A-1/ADM cells treated with ABCG2/BCRP-FITC was 9.8%,39.84 times higher than that of SPC-A-1 cells;it showed significant difference between the ABCG2/BCRP-FITC group and the SPC-A-1/ADM group(t=9.120,P=0.005) and the SPC-A-1/ADM group(t=8.257,P= 0.006).The positive rate of group B cells was 684 times that of group A cells,and the difference was statistically significant(t=11.235,P=0.001),and the fluorescence intensity of group B cells was strong.The average tumorigenic volume of the mice inoculated with SPC-A-1 cells,group A cells,and group B cells was(6.96±1.82),(6.70±2.55),and(9.17±2.41) mm 3,respectively.Among them,group B was the highest,but there was no significant difference among these three groups(F=2.362,P=0.086).The tumorigenic rate of group B cells was 75.00%,which was significantly higher than that of SPC-A-1 cells and group A cells(F=19.780,P=0.002). Conclusion ABCG2 cells from human lung adenocarcinoma SPC-A-1/ADM cell line can be isolated by ABCG2 antibody combined with immunomagnetic beads sorting method,and the tumor formation rate in nude mice can be observed to explore the identification and biological characterization of lung cancer stem cells.

摘要

目的探讨肺癌干细胞的筛选方法及生物学特性。方法从SPC-A-1/阿霉素 (ADM) 中筛选ABCG2 + 和ABCG2-细胞。ADM诱导的细胞系,并通过流式细胞术和裸鼠移植分析ABCG2 + 和ABCG2-细胞的体内致瘤性。结果SPC-A-1 细胞的平均荧光强度为 (1.001 ± 0.014)× 10 2,明显低于SPC-A-1/ADM细胞 [(10.257 ± 0.023) × 10 2 ](t = 17.320,P = 0.001); ABCG2/BCRP-FITC治疗组与SPC-A-1 对照组间差异亦有统计学意义 (t = 5.269,P = 0.021) 和SPC-A-1 对照组 (t = 6.869,P = 0.012)SPC-A-1/ADM细胞对照组和SPC-A-1/ADM细胞同型对照组 (t = 8.112,P = 0.015)。ABCG2/BCRP-FITC处理SPC-A-1/ADM细胞的阳性率为 9.8%,是SPC-A-1 细胞的 39.84 倍; ABCG2/BCRP-FITC组与SPC-A-1/ADM组比较,差异有统计学意义 (t = 9.120,P = 0.005)。和SPC-A-1/ADM组 (t = 8.257,P = 0.006)。B组细胞阳性率为A组细胞的 684 倍,差异有统计学意义 (t = 11.235,P = 0.001),且B组细胞的荧光强度较强。接种SPC-A-1 细胞的小鼠的平均致瘤体积,组A、B组细胞分别为 (6.96 ± 1.82) 、 (6.70 ± 2.55) 、 (9.17 ± 2.41) mm 3。其中B组最高,但 3 组间差异无统计学意义 (F = 2.362,P = 0.086)。B组细胞的致瘤率为 75.00%,显著高于SPC-A-1 细胞和A组细胞 (F = 19.780,P = 0.002)。结论ABCG2 抗体结合免疫磁珠分选法可从人肺腺癌SPC-A-1/ADM细胞系分离ABCG2 细胞,并观察裸鼠体内成瘤率,探讨肺癌干细胞的鉴定及生物学特性。

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影响因子:6.93
发表时间:2020-01-15
DOI:10.1002/ijc.32532
作者列表:["Hata A","Nakajima T","Matsusaka K","Fukuyo M","Morimoto J","Yamamoto T","Sakairi Y","Rahmutulla B","Ota S","Wada H","Suzuki H","Matsubara H","Yoshino I","Kaneda A"]

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影响因子:6.93
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DOI:10.1002/ijc.32530
作者列表:["Zhang L","Yang Y","Chai L","Bu H","Yang Y","Huang H","Ran J","Zhu Y","Li L","Chen F","Li W"]

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肺肿瘤方向

肺肿瘤,又叫支气管肺癌,是常见的恶性肿瘤之一。肺肿瘤的治疗为包括手术、中药、放疗、化疗及免疫等多学科的综合治疗。

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