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Differential miRNA expression analysis of extracellular vesicles from brain microvascular pericytes in spontaneous hypertensive rats.

自发性高血压大鼠脑微血管周细胞胞外囊泡的差异 miRNA 表达分析。

  • 影响因子:2.22
  • DOI:10.1007/s10529-019-02788-x
  • 作者列表:"Wu Q","Yuan X","Li B","Yang J","Han R","Zhang H","Xiu R
  • 发表时间:2020-03-01
Abstract

OBJECTIVE:This study is to explore the exact roles of extracellular vesicle (EVs) miRNAs from brain microvascular pericytes in the pathogenesis of hypertension. RESULTS:Forty-eight significantly differentially expressed miRNAs were identified, of which 17 were found to be upregulated and 31 were found to be downregulated in brain microvascular pericytes of spontaneous hypertensive rats, compared with that of normotension Wistar Kyoto rats. The GO enrichment analysis verified that the target genes were enriched in signaling pathways and molecular functions, such as metal ion binding, nucleotide binding and ATP binding. The KEGG analysis indicated that the target genes were enriched in Linoleic acid, alpha-linolenic acid and sphingolipid metabolism pathways. CONCLUSIONS:Several EV derived miRNAs, such as miR-21-5p, let-7c-5p and let-7a-5p, were found to be abnormally expressed in brain microvascular pericytes obtained from spontaneous hypertensive rats, compared with that of normotension Wistar Kyoto rats. The results of our research provide more insights into the functional link between brain microvascular pericytes and the pathogenesis of hypertension.

摘要

目的: 本研究旨在探讨脑微血管周细胞胞外囊泡 (EVs) miRNAs 在高血压发病机制中的确切作用。 结果: 鉴定出 48 个显著差异表达的 miRNAs,其中在自发性高血压大鼠脑微血管周细胞中发现 17 个上调,31 个下调, 与正常血压 Wistar 京都大鼠比较。GO 富集分析验证了靶基因在信号通路和分子功能上的富集,如金属离子结合、核苷酸结合和 ATP 结合。KEGG 分析表明,目的基因富集于亚油酸、 α-亚麻酸和鞘脂代谢途径。 结论: 与正常血压状态的 Wistar Kyoto 大鼠相比,自发性高血压大鼠脑微血管周细胞中存在 miR-21-5p 、 let-7c-5p 、 let-7a-5p 等 EV 来源的 miRNAs 异常表达。我们的研究结果为脑微血管周细胞与高血压发病机制之间的功能联系提供了更多的见解。

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