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Tumor promoting effects of glucagon receptor: a promising biomarker of papillary thyroid carcinoma via regulating EMT and P38/ERK pathways.

胰高血糖素受体的肿瘤促进作用: 通过调节EMT和P38/ERK通路成为甲状腺乳头状癌的一个有前途的生物标志物。

  • 影响因子:2.15
  • DOI:10.1007/s13577-019-00284-y
  • 作者列表:"Jiang HC","Chen XR","Sun HF","Nie YW
  • 发表时间:2020-01-01
Abstract

:Glucagon is a crucial hormone involved in the maintenance of glucose homeostasis. Large efforts to define the role of glucagon receptor (GCGR) have been continuously made in recent years, but it is still incomplete about its function and mechanism. We performed this study to verify its potential impacts on papillary thyroid carcinoma (PTC) progression. Correlation between GCGR expression and PTC was elaborated using The Cancer Genome Atlas (TCGA) database. The Kaplan-Meier method was used to analyze the connection between GCGR expression and prognosis of PTC patients. GCGR expression was measured by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analysis; simultaneously, cell viability was elucidated using cell proliferation and colony formation assays following siRNAs strategy. Transwell analyses were conducted to measure the invasion and migration of PTC cells. Flow cytometry analysis was conducted to examine apoptotic ability. The cAMP ELISA kit was employed to measure the cAMP level in PTC cells. Our data determined that the expression level of GCGR was increased in PTC tissues and cells in contrast to normal tissues and Nthy-ori 3-1, respectively. Up-regulated GCGR expression was linked with the lower survival rate in patients with PTC. Functional analysis in vitro suggested that GCGR knockdown attenuated PTC cell proliferation, colony formation, invasion, and migration whilst intensified apoptosis. Down-regulated GCGR was able to increase cAMP level. Furthermore, reduction of GCGR could result in the inactivation of epithelial-mesenchymal transition (EMT) and P38/ERK pathways. In conclusion, the findings of this study disclosed that GCGR promoted PTC cell behaviors by mediating the EMT and P38/ERK pathways, serving as a potential diagnostic and prognostic biomarker as well as therapeutic target for PTC.

摘要

: 胰高血糖素是参与维持葡萄糖稳态的关键激素。近年来,关于胰高血糖素受体 (GCGR) 作用的研究不断深入,但其作用机制尚不完全清楚。我们进行了这项研究,以验证其对乳头状甲状腺癌 (PTC) 进展的潜在影响。使用癌症基因组图谱 (TCGA) 数据库阐述了GCGR表达和PTC之间的相关性。采用Kaplan-Meier法分析GCGR表达与PTC患者预后的关系。通过定量实时聚合酶链反应 (qRT-PCR) 和蛋白质印迹分析测量GCGR表达; 同时,使用按照sirna策略的细胞增殖和集落形成测定法阐明细胞活力。进行Transwell分析以测量PTC细胞的侵袭和迁移。进行流式细胞术分析以检查凋亡能力。使用cAMP ELISA试剂盒测量PTC细胞中的cAMP水平。我们的数据确定,与正常组织和Nthy-ori 3-1相比,GCGR的表达水平在PTC组织和细胞中分别增加。GCGR表达上调与PTC患者较低的生存率有关。体外功能分析表明,GCGR敲除减弱PTC细胞增殖、集落形成、侵袭和迁移,同时加剧凋亡。下调的GCGR能够增加cAMP水平。此外,GCGR的减少可导致上皮-间质转化 (EMT) 和P38/ERK途径的失活。总之,本研究的发现揭示了GCGR通过介导EMT和P38/ERK途径促进PTC细胞行为,作为潜在的诊断和预后生物标志物以及PTC的治疗靶标。

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作者列表:["Nishino M","Krane JF"]

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影响因子:2.32
发表时间:2020-01-01
来源期刊:The Laryngoscope
DOI:10.1002/lary.27947
作者列表:["Ji T","Chen J","Mou J","Ni X","Guo Y","Zhang J","Wang S","Wang W","Zhang X","Tai J"]

METHODS:OBJECTIVES/HYPOTHESIS:The definition of large-volume pathologic N1 metastases has been changed in the 2017 version 2 of the National Comprehensive Cancer Network guidelines, leading to a controversy over the optimal surgical approach selection for patients with biopsy-proven papillary thyroid carcinoma (PTC). The aim of this study was to investigate the therapeutic efficacy of total thyroidectomy (TT) and thyroid lobectomy (TL) for these patients. STUDY DESIGN:Retrospective population-based database analysis. METHODS:A total of 906 consecutive PTC patients with pathologic N1 metastases (>5 involved nodes with metastases ≤5 mm in the largest dimension) were retrieved from the Surveillance, Epidemiology, and End Results database, and divided into two groups (≤2 mm, >2-5 mm) based on the size of the extent of disease. Overall survival (OS) was then compared between patients treated with TT and TL, followed by Cox proportional hazards regression analysis to explore multiple prognostic factors. RESULTS:OS favored TT compared with TL in patients with more than five involved nodes and metastases >2 to 5 mm in the largest dimension (P < .05). Cox analysis showed that the TL was not an independent factor associated with poorer OS than TT in these patients (P > .05). CONCLUSIONS:TT showed better survival than TL for patients with more than five involved nodes and metastases >2 to 5 mm in the largest dimension. For patients with more than five involved nodes and metastases ≤2 mm in the largest dimension, either TT or TL can be recommended because there was no difference in survival. LEVEL OF EVIDENCE:NA Laryngoscope, 130:269-273, 2020.

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影响因子:2.09
发表时间:2020-01-01
DOI:10.2174/1573399815666190417145440
作者列表:["Goes LG","da Luz Eltchechem C","Wouk J","Malfatti CRM","da Silva LA"]

METHODS:BACKGROUND:Obesity, diabetes mellitus may be related to the health, the relationship and the physiological capacity of the production of thyroid hormones (TH), triiodothyronine (T3) and thyroxine (T4). OBJECTIVES:The main aims of this review are to describe the relationship between obesity, appetite, weight management, hormonal mechanisms of diabetes mellitus and hypothyroidism post-bariatric surgery. METHODOLOGY:An in-depth literature search was conducted to identify scientific studies, which analyzed the correlation between diabetes mellitus and hypothyroidism post-bariatric surgery. RESULTS:Bariatric surgery decreases hypothyroidism, reduces the need for pharmacological action (such as levothyroxine), controls the weight and body fat and increases the sensitivity to leptin and insulin. CONCLUSION:The reduction of the stomach and intestine by bariatric surgery is an evolutionary and beneficial action, because it may lead to a drastic decrease on numbers of conditions such as diabetes, obesity, hypothyroidism, and others. Thus, new studies should also focus on patients' post-operatory conditions, such as lifetime, regulation and functioning of organs after reduced nutrition, and consumption and delivery of nutrients to health maintenance.

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甲状腺疾病方向

主要分为内科治疗的甲状腺疾病和外科治疗的甲状腺疾病两大类。内科治疗的甲状腺疾病主要包括甲状腺功能亢进症和甲状腺炎症。外科治疗的甲状腺疾病包括甲状腺肿和甲状腺肿瘤。

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