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Levels of pro- and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis.

伴或不伴牙龈炎的囊性纤维化患者促炎和抗炎细胞因子水平。

  • 影响因子:3.45
  • DOI:10.1016/j.cyto.2020.154987
  • 作者列表:"Duruel O","Berker E","Özşin-Özler C","Gharibzadeh-Hızal M","Gürpınar Ö","Eryılmaz-Polat S","Ataman-Duruel ET","Tan Ç","Karabulut E","Tekçiçek M","Eser ÖK","Kiper N","Tezcan İ
  • 发表时间:2020-03-01
Abstract

BACKGROUND:Inflammatory periodontal diseases are caused by interaction between gram negative, anaerobic bacteria and host response. Persistent infection of Pseudomonas aeruginosa in cystic fibrosis (CF) patients also cause increased pro-inflammatory response and the imbalance of pro- and anti-inflammatory response in brochoalveolar lavage fluid which leads to destruction of lungs. The aim of this study is to evaluate periodontal status of CF patients, to measure level of cytokines and biochemical molecules in gingival crevicular fluid (GCF), and to detect presence of P. aeruginosa in dental plaque samples. MATERIALS AND METHODS:GCF samples were collected from 41 CF patients and 39 healthy (non-CF) subjects. Interleukin (IL)-1ß, IL-17, IL-10, human neutrophil elastase (HNE), cystic fibrosis transmembrane regulator (CFTR) protein, and human β-defensin-1 (HBD1) in GCF were evaluated by ELISA method. Dental plaque samples were collected from 18 CF patients with history of P. aeruginosa colonization and 15 non-CF subjects. Presence of P. aeruginosa was evaluated by using conventional culture methods and molecular methods. RESULTS:Levels of IL-1ß, HNE, and HBD1 in CF patients were significantly higher than non-CF subjects. However, IL-10 level was significantly lower in CF patients. Increased pro-inflammatory (IL-1ß) and decreased anti-inflammatory (IL-10) cytokine levels were observed in GCF samples from CF patients, irrespective of their periodontal status. P. aeruginosa were detected in four samples of 18 CF patients, and all were negative in non-CF group. CONCLUSIONS:As a result of this study, CF coexists increasing pro-inflammatory and decreasing anti-inflammatory response locally. Due to increasing pro-inflammation, CF patients should be followed-up more often than non-CF children.

摘要

背景: 炎症性牙周疾病是由革兰氏阴性菌、厌氧菌和宿主反应相互作用引起的。囊性纤维化 (CF) 铜绿假单胞菌持续感染患者还会引起促炎反应增加,以及支气管肺泡灌洗液中促炎和抗炎反应的失衡,从而导致肺的破坏。本研究的目的是评估 CF 患者的牙周状况,测量龈沟液 (GCF) 中细胞因子和生化分子的水平,并检测 P 的存在。牙菌斑样本中的铜绿。 材料和方法: 收集 41 例 CF 患者和 39 例健康 (非 CF) 受试者的 GCF 样本。白细胞介素 (IL)-1 ß 、 IL-17 、 IL-10 、人中性粒细胞弹性蛋白酶 (HNE) 、囊性纤维化跨膜调节因子 (CFTR) 蛋白和人 β-防御素-1 (HBD1) 用 ELISA 法对 GCF 进行评价。收集 18 例有铜绿假单胞菌定植史的 CF 患者和 15 例非 CF 受试者的牙菌斑样本。使用常规培养方法和分子方法评价铜绿假单胞菌的存在。 结果: CF 患者的 il-1 ß 、 HNE 和 HBD1 水平显著高于非 CF 受试者。然而,CF 患者 IL-10 水平显著降低。在 CF 患者的 GCF 样本中观察到促炎 (il-1 ß) 和抗炎 (IL-10) 细胞因子水平的增加,而不管其牙周状况如何。18 例 CF 患者的 4 份标本检出铜绿假单胞菌,非 CF 组均为阴性。 结论: 作为本研究的结果,CF 在局部同时存在促炎症反应增加和抗炎反应降低。由于促炎症反应增加,CF 患者应该比非 CF 儿童更经常地随访。

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翻译标题与摘要 下载文献
影响因子:4.40
发表时间:2020-01-01
DOI:10.1007/s00262-019-02431-8
作者列表:["Shibaki, Ryota","Murakami, Shuji","Matsumoto, Yuji","Yoshida, Tatsuya","Goto, Yasushi","Kanda, Shintaro","Horinouchi, Hidehito","Fujiwara, Yutaka","Yamamoto, Nobuyuki","Kusumoto, Masahiko","Yamamoto, Noboru","Ohe, Yuichiro"]

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翻译标题与摘要 下载文献
影响因子:4.04
发表时间:2020-01-25
来源期刊:New biotechnology
DOI:10.1016/j.nbt.2019.08.006
作者列表:["Sousa SA","Soares-Castro P","Seixas AMM","Feliciano JR","Balugas B","Barreto C","Pereira L","Santos PM","Leitão JH"]

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