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Nanograss sensor for selective detection of Pseudomonas aeruginosa by pyocyanin identification in airway samples.

通过气道样本中的绿脓杆菌鉴定选择性检测铜绿假单胞菌的 Nanograss 传感器。

  • 影响因子:2.34
  • DOI:10.1016/j.ab.2020.113586
  • 作者列表:"Alatraktchi FA","Dimaki M","Støvring N","Johansen HK","Molin S","Svendsen WE
  • 发表时间:2020-01-22
Abstract

:Pyocyanin is a virulence factor solely produced by the pathogen Pseudomonas aeruginosa. Pyocyanin is also a redox active molecule that can be directly detected by electrochemical sensing. A nanograss (NG) based sensor for sensitive quantification of pyocyanin in sputum samples from cystic fibrosis (CF) patients is presented here. The NG sensors were custom made in a cleanroom environment by etching nanograss topography on the electrode surface followed by depositing 200 nm gold. The NG sensors were utilized for amperometric quantification of pyocyanin in spiked hypertonic saline samples, resulting in a linear calibration curve with a R2 value of 0.9901 and a limit of detection of 172 nM. The NG sensors were applied in a small pilot test on five airway samples from five CF patients. The NG sensor was capable of identifying P. aeruginosa in the airway samples in 60 s without any sample pretreatment.

摘要

: 绿脓杆菌素是由病原菌铜绿假单胞菌单独产生的毒力因子。绿脓菌素也是一种氧化还原活性分子,可以通过电化学传感直接检测。本文介绍了一种基于 nanograss (NG) 的传感器,用于敏感定量囊性纤维化 (CF) 患者痰液样本中的绿脓杆菌素。NG 传感器是在洁净室环境中定制的,方法是在电极表面蚀刻纳米草形貌,然后沉积 200 nm 金。使用 NG 传感器对加标高渗盐水样品中的绿脓液进行安培定量,得到线性校准曲线,R2 值为 0.9901,检测限为 172 nM。将 NG 传感器应用于 5 例 CF 患者的 5 个气道样本的小型中试。NG 传感器能够在 60 s 内识别气道样本中的铜绿假单胞菌,无需任何样本预处理。

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作者列表:["Bernstein DM","Toth B","Rogers RA","Kling DE","Kunzendorf P","Phillips JI","Ernst H"]

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影响因子:4.04
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DOI:10.1042/BST20191010
作者列表:["Zaragosi LE","Deprez M","Barbry P"]

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