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Adipose Tissue-Derived Mesenchymal Stem Cells Protect Against Amiodarone-Induced Lung Injury in Rats.

脂肪组织来源的间充质干细胞对胺碘酮诱导的大鼠肺损伤的保护作用。

  • 影响因子:2.17
  • DOI:10.1007/s12010-020-03227-8
  • 作者列表:"Radwan SM","Ghoneim D","Salem M","Saeed M","Saleh Y","Elhamy M","Wael K","Shokair O","Wahdan SA
  • 发表时间:2020-01-17
Abstract

:Pulmonary fibrosis (PF) is a progressive and irreversible lung disease, characterized by poor prognosis with limited treatment options. Mesenchymal stem cells (MSCs) are multi-potent cells having the ability to self-renew and differentiate into multiple tissues, thus considered a novel treatment option. The present study aimed to investigate the possible antifibrotic effect of undifferentiated adipose tissue-derived mesenchymal stem cell (AD-MSC) therapy on PF experimentally induced in rats using amiodarone (AMD). AMD (30 mg/kg) was given orally, once daily for 12 consecutive weeks to induce lung fibrosis. Following the confirmation of lung damage with histopathological examination, AD-MSCs (2 × 106 and 4 × 106 undifferentiated MSCs) were injected once intravenously, followed by 2 months for treatment. AMD induced focal fibroblastic cells proliferation in the peribronchiolar tissue, as well as in between the collapsed emphysematous alveoli. Also, AMD significantly increased serum and lung homogenate fibroblast growth factor-7 (FGF7), Clara cell protein-16 (CC16), and cytokeratin -19 (CK19) levels, as well as the expression of both iNOS and NFкB in the lung alveoli. Moreover, AMD caused excessive collagen deposition and increased alpha smooth muscle actin (α-SMA) expression. All findings significantly regressed on stem cell therapy in both doses, with superior effect of the high dose, providing evidence that adipose tissue-derived MSCs could be a promising approach for the treatment of PF. Graphical Abstract.

摘要

: 肺纤维化 (PF) 是一种进行性和不可逆的肺部疾病,其特点是预后不良,治疗选择有限。间充质干细胞 (MSCs) 是多能细胞,具有自我更新和分化为多个组织的能力,因此被认为是一种新的治疗选择。本研究旨在探讨未分化脂肪组织来源的间充质干细胞 (AD-MSC) 治疗胺碘酮 (AMD) 诱导的大鼠 PF 的可能抗纤维化作用。给予 AMD (30 mg/kg) 口服,每日 1 次,连续 12 周诱导肺纤维化。经组织病理学检查证实为肺损伤后,静脉注射 AD-MSCs (2 × 106 和 4 × 106 未分化 MSCs) 1 次,然后治疗 2 个月。AMD 在细支气管周围组织以及塌陷的肺气肿肺泡之间诱导局灶性成纤维细胞增殖。同样,AMD 显著增加血清和肺匀浆成纤维细胞生长因子-7 (FGF7) 、克拉拉细胞蛋白-16 (CC16) 和细胞角蛋白-19 (CK19) 水平, 以及肺泡中 iNOS 和 nf к b 的表达。此外,AMD 引起胶原过度沉积,α 平滑肌肌动蛋白 (α-SMA) 表达增加。所有结果在两种剂量的干细胞治疗中均显著消退,具有高剂量的优越效果,提供了脂肪组织来源的 MSCs 可能是治疗 PF 的一种有前途的方法的证据。图形摘要。

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翻译标题与摘要 下载文献
影响因子:4.40
发表时间:2020-01-01
DOI:10.1007/s00262-019-02431-8
作者列表:["Shibaki, Ryota","Murakami, Shuji","Matsumoto, Yuji","Yoshida, Tatsuya","Goto, Yasushi","Kanda, Shintaro","Horinouchi, Hidehito","Fujiwara, Yutaka","Yamamoto, Nobuyuki","Kusumoto, Masahiko","Yamamoto, Noboru","Ohe, Yuichiro"]

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翻译标题与摘要 下载文献
影响因子:4.04
发表时间:2020-01-25
来源期刊:New biotechnology
DOI:10.1016/j.nbt.2019.08.006
作者列表:["Sousa SA","Soares-Castro P","Seixas AMM","Feliciano JR","Balugas B","Barreto C","Pereira L","Santos PM","Leitão JH"]

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