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Fibroblast growth factor 10 alleviates particulate matter-induced lung injury by inhibiting the HMGB1-TLR4 pathway.

成纤维细胞生长因子 10 通过抑制肺损伤途径减轻颗粒物诱导的 HMGB1-TLR4。

  • 影响因子:5.5150
  • DOI:10.18632/aging.102676
  • 作者列表:"Liu L","Song C","Li J","Wang Q","Zhu M","Hu Y","Chen J","Chen C","Zhang JS","Dong N","Chen C
  • 发表时间:2020-01-20
Abstract

:Exposure to particulate matter (PM) is associated with increased incidence of respiratory diseases. The present study aimed to investigate the roles of fibroblast growth factor 10 (FGF10) in PM-induced lung injury. Mice were intratracheally instilled with FGF10 or phosphate-buffered saline at one hour before instillation of PM for two consecutive days. In addition, the anti-inflammatory impact of FGF10 in vitro and its effect on the high-mobility group box 1 (HMGB1)-toll-like receptor 4 (TLR4) pathway was investigated. It was found that PM exposure is associated with increased inflammatory cell infiltration into the lung and increased vascular protein leakage, while FGF10 pretreatment attenuated both of these effects. FGF10 also decreased the PM-induced expression of interleukin (IL)-6, IL-8, tumor necrosis factor-α and HMGB1 in murine bronchoalveolar lavage fluid and in the supernatants of human bronchial epithelial cells exposed to PM. FGF10 exerted anti-inflammatory and cytoprotective effects by inhibiting the HMGB1-TLR4 pathway. These results indicate that FGF10 may have therapeutic values for PM-induced lung injury.

摘要

: 颗粒物 (PM) 暴露与呼吸系统疾病发病率增加有关。本研究旨在探讨成纤维细胞生长因子 10 (FGF10) 在 PM 诱导的肺损伤中的作用。小鼠在滴注 PM 前 1 小时气管内滴注 FGF10 或磷酸盐缓冲盐水,连续两天。此外,研究了 FGF10 的体外抗炎作用及其对高迁移率族蛋白 b1 (HMGB1)-toll 样受体 4 (TLR4) 通路的影响。发现 PM 暴露与肺内炎症细胞浸润增加和血管蛋白渗漏增加有关,而 FGF10 预处理减弱了这两种作用。FGF10 还降低 PM 诱导的白细胞介素 (IL)-6 、 IL-8 、小鼠支气管肺泡灌洗液和 PM 暴露的人支气管上皮细胞上清液中的肿瘤坏死因子-α 和 HMGB1。FGF10 通过抑制 HMGB1-TLR4 途径发挥抗炎和细胞保护作用。这些结果表明 FGF10 可能对 PM 诱导的肺损伤有治疗价值。

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作者列表:["Bernstein DM","Toth B","Rogers RA","Kling DE","Kunzendorf P","Phillips JI","Ernst H"]

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DOI:10.1042/BST20191010
作者列表:["Zaragosi LE","Deprez M","Barbry P"]

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