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Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injury.

在暴露于香烟烟雾期间,piperum胺的作用可减少炎症和肺损伤。

  • 影响因子:2.74
  • DOI:10.1016/j.pupt.2020.101896
  • 作者列表:"Sant'Ana Leal M","Souza HR","Possebon L","Cornélio ML","Riffo-Vasquez Y","Girol AP","Oliani SM
  • 发表时间:2020-01-24
Abstract

:Chronic obstructive pulmonary disease (COPD) is related to smoking and anti-inflammatory therapy is indicated. Among the mediators with anti-inflammatory properties, we highlight piperlongumine (PL), an alkaloid/amide of Piper longum. Here we evaluated the PL administration on an experimental model of respiratory inflammation resulting from exposure to cigarette smoke. Male Balb/c mice were exposed to burning of 10 commercial cigarettes, 2x/day, for five weeks on specific equipment. PL efficacy was evaluated in control, exposed to smoke without treatment and PL treated (2.0 mg/kg, 3x/week) groups. Animals were weighed and plethysmographic analyses performed at the end of the exposure protocol. Inflammatory cells were evaluated in the bronchoalveolar lavage (BAL) and hemoglobin and glucose in the blood. Lung fragments were processed for histopathological studies and AnxA1, COX-2, NF-kB and neutrophil elastase expressions. Plethysmography revealed that PL maintained pulmonary frequency, volume and ventilation parameters similar to controls, with respiratory volume reduction compared to untreated animals. Final weight was reduced in both exposed groups. PL decreased hemoglobin concentration, attenuated the reduction of glucose levels and reduced influx of lymphocytes, neutrophils and macrophages in BAL. Histopathologically occured infiltration of inflammatory cells, increase of the interalveolar septa and intra-alveolar spaces in untreated animals. But, PL administration recovered lung tissues and, immunohistochemically, promoted increased expression of AnxA1 and reduction of COX-2, NF-kB and neutrophil elastase. Together the results indicate that PL attenuates systemic and pulmonary inflammatory changes, partially by modulating the expression the endogenous AnxA1, and may represent a promising therapy in preventing the inflammation induced by cigarette smoke.

摘要

: 慢性阻塞性肺疾病 (COPD) 与吸烟有关,有抗炎治疗指征。在具有抗炎特性的介质中,我们突出了 piperum碱 (PL),Piper Piper 的生物碱/酰胺。在这里,我们在暴露于香烟烟雾导致的呼吸道炎症的实验模型上评价了 PL 给药。雄性 Balb/c 小鼠在特定设备上暴露于 10 支商业香烟的燃烧,2x/天,持续 5 周。在对照组、暴露于未治疗的烟雾和 PL 治疗 (2.0 mg/kg,3x/周) 组中评价 PL 疗效。动物称重,并在暴露方案结束时进行体积描记分析。评估支气管肺泡灌洗 (BAL) 中的炎症细胞以及血液中的血红蛋白和葡萄糖。处理肺碎片进行组织病理学研究和 AnxA1 、 COX-2 、 NF-kB 和中性粒细胞弹性蛋白酶表达。体积描记术显示,PL 维持的肺频率、容量和通气参数与对照组相似,与未治疗动物相比,呼吸容量减少。两种暴露组的最终体重均降低。PL 降低血红蛋白浓度,减弱 BAL 中葡萄糖水平的降低,减少淋巴细胞、中性粒细胞和巨噬细胞的内流。未治疗动物在组织病理学上出现炎性细胞浸润、肺泡间隔和肺泡内间隙增加。但是,PL 给药恢复了肺组织,免疫组化显示,AnxA1 的表达增加,COX-2 、 NF-kB 和中性粒细胞弹性蛋白酶的减少。总之,这些结果表明 PL 减轻了全身和肺部的炎症变化,部分是通过调节内源性 AnxA1 的表达,可能是预防香烟烟雾诱导的炎症的一种有前途的疗法。

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影响因子:3.94
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DOI:10.1016/j.taap.2019.114847
作者列表:["Bernstein DM","Toth B","Rogers RA","Kling DE","Kunzendorf P","Phillips JI","Ernst H"]

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影响因子:4.04
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DOI:10.1042/BST20191010
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