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GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.

基于 GC-MS 的肺组织代谢组学与网络药理学相结合,揭示了蒲地蓝消炎口服液对 LPS 诱导小鼠肺损伤的可能保护机制。

  • 影响因子:3.78
  • DOI:10.1016/j.biopha.2020.109833
  • 作者列表:"Tian G","Li C","Zhai Y","Xu J","Feng L","Yao W","Bao B","Zhang L","Ding A
  • 发表时间:2020-01-17
Abstract

:Pudilan Xiaoyan Oral Liquid (PDL) originated from "Pudilan" Classic Recipe of traditional Chinese medicine is one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia. PDL has been used clinically for treating inflammatory diseases of the respiratory tract. However, due to the complex composition of PDL, its potential anti-inflammation and the mechanism remain unknown. To identify the mechanism of the PDL in the treatment of lipopolysaccharide (LPS)-induced lung injury of mice. The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL. The approach of GC-MS metabolomics was used to find more significant metabolites, and the metabolic pathways were enriched through MetaboAnalyst. Then network analysis was applied to visualize the protein related to the important metabolites, merging into a protein-metabolite network via Cytoscape. The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum. 11 potential metabolites were identified in lung tissue through metabolomics, which were significantly regulated to recover by PDL treatment. The correlated network was constructed by integrating potential metabolites and pathways. Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL. These results demonstrated that PDL might prevent the pathological process of lung injury through regulating the disturbed protein-metabolite network.

摘要

: 蒲地蓝消炎口服液 (PDL) 源于 “蒲地蓝味” 中药经典方剂,是《中国药典》记载的一类消炎中成药。PDL 在临床上用于治疗呼吸道炎症性疾病。然而,由于 PDL 的复杂组成,其潜在的抗炎作用及其机制仍然未知。探讨 PDL 治疗脂多糖 (LPS) 诱导的小鼠肺损伤的作用机制。建立小鼠肺损伤模型,检测血清生化指标和组织病理学变化,探讨 PDL 的作用。利用 GC-MS 代谢组学的方法寻找更显著的代谢产物,并通过代谢分析富集代谢途径。然后应用网络分析可视化与重要代谢物相关的蛋白质,通过 Cytoscape 合并成蛋白质-代谢物网络。PDL 治疗可减轻 LPS 诱导的肺组织病理损伤,降低血清中 IL-10 、 TNF-α 和 NF-κ b 等促炎症介质。通过代谢组学在肺组织中鉴定出 11 种潜在代谢产物,经 PDL 治疗后这些代谢产物恢复明显。通过整合潜在的代谢产物和通路构建相关网络。选择天冬氨酸和 l-半胱氨酸作为关键代谢产物,推测 IL4I1 和 ASPA 等相关蛋白是利用 PDL 治疗 LPS 诱导的肺损伤的潜在靶点。这些结果表明 PDL 可能通过调节紊乱的蛋白质-代谢物网络来阻止肺损伤的病理过程。

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DOI:10.1016/j.taap.2019.114847
作者列表:["Bernstein DM","Toth B","Rogers RA","Kling DE","Kunzendorf P","Phillips JI","Ernst H"]

METHODS::The interim results from this 90-day multi-dose, inhalation toxicology study with life-time post-exposure observation has shown an important fundamental difference in persistence and pathological response in the lung between brake dust derived from brake-pads manufactured with chrysotile, TiO2 or chrysotile alone in comparison to the amphiboles, crocidolite and amosite asbestos. In the brake dust exposure groups no significant pathological response was observed at any time. Slight macrophage accumulation of particles was noted. Wagner-scores, were from 1 to 2 (1 = air-control group) and were similar to the TiO2 group. Chrysotile being biodegradable, shows a weakening of its matrix and breaking into short fibers & particles that can be cleared by alveolar macrophages and continued dissolution. In the chrysotile exposure groups, particle laden macrophage accumulation was noted leading to a slight interstitial inflammatory response (Wagner-score 1-3). There was no peribronchiolar inflammation and occasional very slight interstitial fibrosis. The histopathology and the confocal analyses clearly differentiate the pathological response from amphibole asbestos, crocidolite and amosite, compared to that from the brake dust and chrysotile. Both crocidolite and amosite induced persistent inflammation, microgranulomas, and fibrosis (Wagner-scores 4), which persisted through the post exposure period. The confocal microscopy of the lung and snap-frozen chestwalls quantified the extensive inflammatory response and collagen development in the lung and on the visceral and parietal surfaces. The interim results reported here, provide a clear basis for differentiating the effects from brake dust exposure from those following amphibole asbestos exposure. The subsequent results through life-time post-exposure will follow.

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DOI:10.1042/BST20191010
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