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Zinc supplementation ameliorates lung injury by reducing neutrophil recruitment and activity.

补锌通过减少中性粒细胞募集和活动来改善肺损伤。

  • 影响因子:4.63
  • DOI:10.1136/thoraxjnl-2019-213357
  • 作者列表:"Wessels I","Pupke JT","von Trotha KT","Gombert A","Himmelsbach A","Fischer HJ","Jacobs MJ","Rink L","Grommes J
  • 发表时间:2020-01-08
Abstract

INTRODUCTION:Zinc is well known for its anti-inflammatory effects, including regulation of migration and activity of polymorphonuclear neutrophils (PMN). Zinc deficiency is associated with inflammatory diseases such as acute lung injury (ALI). As deregulated neutrophil recruitment and their hyper-activation are hallmarks of ALI, benefits of zinc supplementation on the development of lipopolysaccharides (LPS)-induced ALI were tested. METHODS:64 C57Bl/6 mice, split into eight groups, were injected with 30 µg zinc 24 hours before exposure to aerosolised LPS for 4 hours. Zinc homoeostasis was characterised measuring serum and lung zinc concentrations as well as metallothionein-1 expression. Recruitment of neutrophils to alveolar, interstitial and intravascular space was assessed using flow cytometry. To determine the extent of lung damage, permeability and histological changes and the influx of protein into the bronchoalveolar lavage fluid were measured. Inflammatory status and PMN activity were evaluated via tumour necrosis factor α levels and formation of neutrophil extracellular traps. The effects of zinc supplementation prior to LPS stimulation on activation of primary human granulocytes and integrity of human lung cell monolayers were assessed as well. RESULTS:Injecting zinc 24 hours prior to LPS-induced ALI indeed significantly decreased the recruitment of neutrophils to the lungs and prevented their hyperactivity and thus lung damage was decreased. Results from in vitro investigations using human cells suggest the transferability of the finding to human disease, which remains to be tested in more detail. CONCLUSION:Zinc supplementation attenuated LPS-induced lung injury in a murine ALI model. Thus, the usage of zinc-based strategies should be considered to prevent detrimental consequences of respiratory infection and lung damage in risk groups.

摘要

简介: 锌以其抗炎作用而闻名,包括调节多形核中性粒细胞 (PMN) 的迁移和活性。锌缺乏与炎症性疾病如急性肺损伤 (ALI) 有关。由于失调的中性粒细胞募集及其过度激活是 ALI 的标志,测试了补锌对脂多糖 (LPS) 诱导的 ALI 发生的益处。 方法: 64 只 C57Bl/6 小鼠分成 8 组,在雾化 LPS 暴露 4 小时前 24 小时注射 30 µ g 锌。测定血清和肺锌浓度以及 metallothionein-1 表达。使用流式细胞术评估中性粒细胞向肺泡、间质和血管内间隙的募集。为了确定肺损伤的程度,测量了通透性和组织学变化以及蛋白流入支气管肺泡灌洗液的情况。通过肿瘤坏死因子 α 水平和中性粒细胞胞外陷阱的形成评价炎症状态和 PMN 活性。还评估了 LPS 刺激前补锌对原代人粒细胞活化和人肺细胞单层完整性的影响。 结果: 在 LPS 诱导的 ALI 前 24 小时注射锌确实显著降低了中性粒细胞向肺的募集,防止了中性粒细胞的过度活跃,从而减轻了肺损伤。利用人类细胞进行的体外研究结果表明,这一发现对人类疾病的可转移性还有待更详细的验证。 结论: 补锌可减轻 LPS 诱导的小鼠 ALI 模型肺损伤。因此,应考虑使用基于锌的策略,以防止危险组中呼吸道感染和肺损伤的有害后果。

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影响因子:3.94
发表时间:2020-01-15
DOI:10.1016/j.taap.2019.114847
作者列表:["Bernstein DM","Toth B","Rogers RA","Kling DE","Kunzendorf P","Phillips JI","Ernst H"]

METHODS::The interim results from this 90-day multi-dose, inhalation toxicology study with life-time post-exposure observation has shown an important fundamental difference in persistence and pathological response in the lung between brake dust derived from brake-pads manufactured with chrysotile, TiO2 or chrysotile alone in comparison to the amphiboles, crocidolite and amosite asbestos. In the brake dust exposure groups no significant pathological response was observed at any time. Slight macrophage accumulation of particles was noted. Wagner-scores, were from 1 to 2 (1 = air-control group) and were similar to the TiO2 group. Chrysotile being biodegradable, shows a weakening of its matrix and breaking into short fibers & particles that can be cleared by alveolar macrophages and continued dissolution. In the chrysotile exposure groups, particle laden macrophage accumulation was noted leading to a slight interstitial inflammatory response (Wagner-score 1-3). There was no peribronchiolar inflammation and occasional very slight interstitial fibrosis. The histopathology and the confocal analyses clearly differentiate the pathological response from amphibole asbestos, crocidolite and amosite, compared to that from the brake dust and chrysotile. Both crocidolite and amosite induced persistent inflammation, microgranulomas, and fibrosis (Wagner-scores 4), which persisted through the post exposure period. The confocal microscopy of the lung and snap-frozen chestwalls quantified the extensive inflammatory response and collagen development in the lung and on the visceral and parietal surfaces. The interim results reported here, provide a clear basis for differentiating the effects from brake dust exposure from those following amphibole asbestos exposure. The subsequent results through life-time post-exposure will follow.

关键词: 暂无
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影响因子:4.04
发表时间:2020-01-10
DOI:10.1042/BST20191010
作者列表:["Zaragosi LE","Deprez M","Barbry P"]

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