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Lung Innate Lymphoid Cell Composition Is Altered in Primary Graft Dysfunction.

原发性移植物功能障碍时肺固有淋巴样细胞组成改变。

  • 影响因子:5.24
  • DOI:10.1164/rccm.201906-1113OC
  • 作者列表:"Monticelli LA","Diamond JM","Saenz SA","Tait Wojno ED","Porteous MK","Cantu E","Artis D","Christie JD
  • 发表时间:2020-01-01
Abstract

:Rationale: Primary graft dysfunction (PGD) is the leading cause of early morbidity and mortality after lung transplantation, but the immunologic mechanisms are poorly understood. Innate lymphoid cells (ILC) are a heterogeneous family of immune cells regulating pathologic inflammation and beneficial tissue repair. However, whether changes in donor-derived lung ILC populations are associated with PGD development has never been examined.Objectives: To determine whether PGD in chronic obstructive pulmonary disease or interstitial lung disease transplant recipients is associated with alterations in ILC subset composition within the allograft.Methods: We performed a single-center cohort study of lung transplantation patients with surgical biopsies of donor tissue taken before, and immediately after, allograft reperfusion. Donor immune cells from 18 patients were characterized phenotypically by flow cytometry for single-cell resolution of distinct ILC subsets. Changes in the percentage of ILC subsets with reperfusion or PGD (grade 3 within 72 h) were assessed.Measurements and Main Results: Allograft reperfusion resulted in significantly decreased frequencies of natural killer cells and a trend toward reduced ILC populations, regardless of diagnosis (interstitial lung disease or chronic obstructive pulmonary disease). Seven patients developed PGD (38.9%), and PGD development was associated with selective reduction of the ILC2 subset after reperfusion. Conversely, patients without PGD exhibited significantly higher ILC1 frequencies before reperfusion, accompanied by elevated ILC2 frequencies after allograft reperfusion.Conclusions: The composition of donor ILC subsets is altered after allograft reperfusion and is associated with PGD development, suggesting that ILCs may be involved in regulating lung injury in lung transplant recipients.

摘要

: 基本原理: 原发性移植物功能障碍 (PGD) 是肺移植后早期发病率和死亡率的主要原因,但免疫机制知之甚少。固有淋巴细胞 (ILC) 是一个异质性的免疫细胞家族,调节病理性炎症和有益的组织修复。然而,供体来源的肺 ILC 人群的变化是否与 PGD 发生相关从未被检查过。目的: 确定慢性阻塞性肺疾病或间质性肺病移植受者的 PGD 是否与同种异体移植物内 ILC 亚群组成的改变相关。方法:我们对肺移植患者进行了一项单中心队列研究,在同种异体移植再灌注之前和之后立即进行了供体组织的外科活检。通过流式细胞术对 18 例患者的供体免疫细胞进行表型表征,以获得不同 ILC 亚群的单细胞分辨率。评估再灌注或 PGD (72 h 内 3 级) ILC 亚群百分比的变化。测量和主要结果: 无论诊断 (间质性肺病或慢性阻塞性肺病) 如何,同种异体移植物再灌注导致自然杀伤细胞频率显著降低,ILC 种群数量有减少的趋势。7 例患者发生 PGD (38.9%),PGD 发生与再灌注后 ILC2 亚群选择性减少相关。相反,无 PGD 的患者在再灌注前表现出显著较高的 ILC1 频率,伴随着同种异体移植物再灌注后 ILC2 频率的升高。结论: 供体 ILC 亚群组成在移植肺再灌注后发生改变,并与 PGD 的发生有关,提示 ILC 可能参与肺移植受者肺损伤的调节。

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影响因子:3.94
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DOI:10.1016/j.taap.2019.114847
作者列表:["Bernstein DM","Toth B","Rogers RA","Kling DE","Kunzendorf P","Phillips JI","Ernst H"]

METHODS::The interim results from this 90-day multi-dose, inhalation toxicology study with life-time post-exposure observation has shown an important fundamental difference in persistence and pathological response in the lung between brake dust derived from brake-pads manufactured with chrysotile, TiO2 or chrysotile alone in comparison to the amphiboles, crocidolite and amosite asbestos. In the brake dust exposure groups no significant pathological response was observed at any time. Slight macrophage accumulation of particles was noted. Wagner-scores, were from 1 to 2 (1 = air-control group) and were similar to the TiO2 group. Chrysotile being biodegradable, shows a weakening of its matrix and breaking into short fibers & particles that can be cleared by alveolar macrophages and continued dissolution. In the chrysotile exposure groups, particle laden macrophage accumulation was noted leading to a slight interstitial inflammatory response (Wagner-score 1-3). There was no peribronchiolar inflammation and occasional very slight interstitial fibrosis. The histopathology and the confocal analyses clearly differentiate the pathological response from amphibole asbestos, crocidolite and amosite, compared to that from the brake dust and chrysotile. Both crocidolite and amosite induced persistent inflammation, microgranulomas, and fibrosis (Wagner-scores 4), which persisted through the post exposure period. The confocal microscopy of the lung and snap-frozen chestwalls quantified the extensive inflammatory response and collagen development in the lung and on the visceral and parietal surfaces. The interim results reported here, provide a clear basis for differentiating the effects from brake dust exposure from those following amphibole asbestos exposure. The subsequent results through life-time post-exposure will follow.

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影响因子:4.04
发表时间:2020-01-10
DOI:10.1042/BST20191010
作者列表:["Zaragosi LE","Deprez M","Barbry P"]

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