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Different macrophage polarization between drug-susceptible and multidrug-resistant pulmonary tuberculosis

药物敏感与耐多药肺结核巨噬细胞的不同极化

  • 影响因子:2.79
  • DOI:10.1186/s12879-020-4802-9
  • 作者列表:"Hyun Jin Cho","Yun-Ji Lim","Jhingook Kim","Won-Jung Koh","Chang-Hwa Song","Min-Woong Kang
  • 发表时间:2020-02-02
Abstract

Abstract Background Macrophages play a key role in the infection process, and alternatively activated macrophages (M2 polarization) play important roles in persistent infection via the immune escape of pathogens. This suggests that immune escape of pathogens from host immunity is an important factor to consider in treatment failure and multidrug-resistant tuberculosis (MDR-TB)/extensively drug-resistant tuberculosis (XDR-TB). In this study, we investigated the association between macrophage polarization and MDR-TB/XDR-TB and the association between macrophage polarization and the anti-TB drugs used. Methods iNOS and arginase-1, a surface marker of polarized macrophages, were quantified by immunohistochemical staining and imaging analysis of lung tissues of patients who underwent surgical treatment for pulmonary TB. Drug susceptibility/resistance and the type and timing of anti-tuberculosis drugs used were investigated. Results The M2-like polarization rate and the ratio of the M2-like polarization rate to the M1-like polarization rate were significantly higher in the MDR-TB/XDR-TB group than in the DS-TB group. The association between a high M2-like polarization rate and MDR-TB/XDR-TB was more pronounced in patients with a low M1-like polarization rate. Younger age and a higher M2-like polarization rate were independent associated factors for MDR-TB/XDR-TB. The M2-like polarization rate was significantly higher in patients who received anti-TB drugs containing pyrazinamide continuously for 4 or 6 weeks than in those who received anti-TB drugs not containing pyrazinamide. Conclusions The M2-like polarization of macrophages is associated with MDR-TB/XDR-TB and anti-TB drug regimens including pyrazinamide or a combination of pyrazinamide, prothionamide and cycloserine.

摘要

【摘要】背景巨噬细胞在感染过程中起着关键作用,交替激活的巨噬细胞 (M2 极化) 通过病原体的免疫逃逸在持续性感染中发挥重要作用。这表明病原体从宿主免疫逃逸是治疗失败和耐多药结核病 (MDR-TB) 的重要考虑因素 /广泛耐药结核病 (XDR-TB)。在这项研究中,我们调查了巨噬细胞极化与 MDR-TB/XDR-TB 之间的关联,以及巨噬细胞极化与所用抗结核药物之间的关联。方法采用免疫组织化学染色和影像学分析方法,对肺结核病患者的肺组织进行极化巨噬细胞表面标记物 iNOS 和 arginase-1 的定量分析。药敏/耐药和抗结核药物使用的类型和时间进行了调查。结果 MDR-TB/XDR-TB 组的 M2-like 极化率和 M2-like 极化率与 M1-like 极化率的比值明显高于 DS-TB 组。高 M2-like 极化率与 MDR-TB/XDR-TB 之间的关联在低 M1-like 极化率的患者中更为明显。低龄和高 M2-like 极化率是 MDR-TB/XDR-TB 的独立相关因素。连续服用含吡嗪酰胺的抗结核药物 4 周或 6 周的患者 M2-like 极化率显著高于不含吡嗪酰胺的抗结核药物的患者。结论巨噬细胞的 M2-like 极化与 MDR-TB/XDR-TB 和抗结核药物包括吡嗪酰胺或吡嗪酰胺、丙硫酰胺和环丝氨酸的组合有关。

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影响因子:3.94
发表时间:2020-01-15
DOI:10.1016/j.taap.2019.114847
作者列表:["Bernstein DM","Toth B","Rogers RA","Kling DE","Kunzendorf P","Phillips JI","Ernst H"]

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影响因子:4.04
发表时间:2020-01-10
DOI:10.1042/BST20191010
作者列表:["Zaragosi LE","Deprez M","Barbry P"]

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