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Low FCMR mRNA expression in leukocytes of patients with Kawasaki disease six months after disease onset.

川崎病患者发病后 6 个月白细胞 FCMR mRNA 低表达。

  • 影响因子:2.88
  • DOI:10.1111/pai.13235
  • 作者列表:"Ling-Sai C","Guo MM","Yan JH","Huang YH","Lo MH","Kuo HC
  • 发表时间:2020-02-19
Abstract

BACKGROUND:Immunoglobulin (Ig) M plays an important role in immune regulation. FCMR-encoded FcμR is a receptor of IgM. Previous research has suggested that Ig M levels may be involved in the coronary artery lesions of Kawasaki syndrome (KD). In this study, we aimed to explore the roles of mRNA expressions of IgM receptors, particularly FCMR, in KD patients. METHODS:We enrolled 60 KD patients and 55 non-KD controls. Whole blood leukocytes were isolated and the mRNA expression for FCMR was determined. Each mRNA consisted of a sample taken before intravenous immunoglobulin (IVIG) was administered (acute, KD1) and those taken at three weeks, six months, and one year later (KD3, KD4, KD5). Paired KD subjects were analyzed from both the acute and convalescent phase (n = 28). RESULTS:After six months and one year of treatment, KD patients still apparently have lower FCMR compared with controls (p = 0.004). FCMR expressions were down-regulated in male patients with KD prior to IVIG administration (p = 0.044). The FCMR of paired KD patients who received IVIG treatments after six months was significantly lower than before undergoing IVIG treatment (p= 0.044). Expressions in the polymorphonuclear leukocytes were similar to those in the peripheral blood mononuclear cells. CONCLUSION:The unique data supported that FCMR is expressed by granulocytes at RNA levels in humans and demonstrated lower FCMR six months after the onset of KD. The findings remind us of the need to track the health of children with KD over the long term, even if we think patients have fully recovered.

摘要

背景: 免疫球蛋白 (Ig) M 在免疫调节中起重要作用。FCMR 编码的 fc μ r 是 IgM 的受体。既往研究提示 Ig M 水平可能参与了川崎综合征 (KD) 的冠状动脉病变。在这项研究中,我们旨在探讨 IgM 受体,特别是 FCMR 的 mRNA 表达在 KD 患者中的作用。 方法: 我们入组了 60 例 KD 患者和 55 例非 KD 对照。分离全血白细胞,测定 FCMR 的 mRNA 表达。每个 mRNA 由静脉注射免疫球蛋白 (IVIG) 给药前 (急性,KD1) 和一年后 (KD3,KD4, KD5)。从急性期和恢复期 (n = 28) 分析配对 KD 受试者。 结果: 川崎病患者治疗 6 个月、 1 年时,其 FCMR 仍明显低于对照组 (p = 0.004)。给予 IVIG 前 KD 男性患者 FCMR 表达下调 (p = 0.044)。6 个月后接受 IVIG 治疗的配对 KD 患者的 FCMR 显著低于接受 IVIG 治疗前 (p = 0.044)。多形核白细胞的表达与外周血单个核细胞相似。 结论: 独特的数据支持 FCMR 在人类粒细胞 RNA 水平表达,并在 KD 发病后 6 个月表现出较低的 FCMR。这些发现提醒我们有必要长期跟踪 KD 儿童的健康状况,即使我们认为患者已经完全康复。

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