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Human adipose-derived mesenchymal stem cells inhibit proliferation and induce apoptosis of human gastric cancer HGC-27 cells

人脂肪间充质干细胞抑制人胃癌 HGC-27 细胞增殖并诱导其凋亡

  • 影响因子:2.61
  • DOI:10.1007/s13205-020-2090-0
  • 作者列表:"Zhao, Jianhong","Zhang, Zilong","Cui, Qingfeng","Zhao, Lina","Hu, Yongjun","Zhao, Subin
  • 发表时间:2020-02-21
Abstract

The aim of this study was to explore the effects of human adipose-derived mesenchymal stem cells (ASCs) on the growth of gastric cancer cells in vivo and vitro and its mechanism. ASCs were isolated from abandoned adipose tissues, and the surface markers were identified by flow cytometry. In vitro experiments, HGC-27 cells cultured in ASCs-conditioned medium (CM) were assigned as the experimental group, while HGC-27 cells cultured in normal medium were as the control group. MTT and colony formation assays were performed to detect cell viability and colony formatting ability, respectively. Annexin-V/PI assay, Western blot, and caspase-3 enzyme activity assay were performed to detect cells apoptosis. The isolated ASCs could be differentiated into adipocytes and osteoblasts in vitro. Flow cytometry showed that CD73 and CD105 were positively expressed in HGC-27 cells. Compared with the mice injected HGC-27 cells only, the tumor formation in mice injected both ASCs and HGC-27 cells was significantly smaller ( P  < 0.05). The colony formation ability in experimental group was 40.09% smaller than control group ( P  < 0.05) and the cell apoptosis rate in experimental group was higher than the control group ( P  < 0.05). Furthermore, the expressions of cleaved PARP, cleaved caspase-3 proteins, and caspase-3 enzyme viability in experimental group were significantly higher than those of control group ( P  < 0.05). In conclusion, ASCs can effectively inhibit the growth of HGC-27 cells by inducing apoptosis.

摘要

本研究旨在探讨人脂肪间充质干细胞 (ASCs) 在体内外对胃癌细胞生长的影响及其机制。从废弃脂肪组织中分离 ASCs,流式细胞仪鉴定表面标志物。体外实验将 ASCs 条件培养液 (CM) 培养的 HGC-27 细胞设为实验组,正常培养液培养的 HGC-27 细胞设为对照组。MTT 和集落形成试验分别检测细胞活力和集落形成能力。Annexin-V/PI 测定、 Western blot 和 caspase-3 酶活性测定检测细胞凋亡。分离的 ASCs 在体外可分化为脂肪细胞和成骨细胞。流式细胞仪检测显示 CD73 和 CD105 在 HGC-27 细胞中呈阳性表达。与仅注射 HGC-27 细胞的小鼠相比,注射 ASCs 和 HGC-27 细胞的小鼠肿瘤形成明显较小 (P  

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DOI:10.1080/09553002.2020.1721609
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影响因子:2.69
发表时间:2020-01-18
DOI:10.1016/j.bbrc.2020.01.048
作者列表:["Li Y","Wang Z","Jin J","Zhu SX","He GQ","Li SH","Wang J","Cai Y"]

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影响因子:2.46
发表时间:2020-01-01
DOI:10.1097/COC.0000000000000609
作者列表:["Appelt AL","Andersen RF","Lindebjerg J","Jakobsen A"]

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