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Indocyanine green based fluorescent polymeric nanoprobes for in vitro imaging.

基于吲哚菁绿的荧光聚合物纳米探针用于体外成像。

  • 影响因子:2.69
  • DOI:10.1002/jbm.b.34410
  • 作者列表:"Ege ZR","Akan A","Oktar FN","Lin CC","Kuruca DS","Karademir B","Sahin YM","Erdemir G","Gunduz O
  • 发表时间:2020-02-01
Abstract

:Indocyanine green (ICG) provides an advantage in the imaging of deep tumors as it can reach deeper location without being absorbed in the upper layers of biological tissues in the wavelengths, which named "therapeutic window" in the tissue engineering. Unfortunately, rapid elimination and short-term stability in aqueous media limited its use as a fluorescence probe for the early detection of cancerous tissue. In this study, stabilization of ICG was performed by encapsulating ICG molecules into the biodegradable polymer composited with poly(l-lactic acid) and poly(ε-caprolactone) via a simple one-step multiaxial electrospinning method. Different types of coaxial and triaxial structure groups were performed and compared with single polymer only groups. Confocal microscopy was used to image the encapsulated ICG (1 mg/mL) within electrospun nanofibers and in vitro ICG uptake by MIA PaCa-2 pancreatic cancer cells. Stability of encapsulated ICG is demonstrated by the in vitro sustainable release profile in PBS (pH = 4 and 7) up to 21 days. These results suggest the potential of the ability of internalization and accommodation of ICG into the pancreatic cell cytoplasm from in vitro implanted ICG-encapsulated multiaxial nanofiber mats. ICG-encapsulated multilayer nanofibers may be promising for the local sustained delivery system to eliminate loss of dosage caused by direct injection of ICG-loaded nanoparticles in systemic administration.

摘要

: 吲哚菁绿 (ICG) 在深部肿瘤成像中提供了优势,因为它可以到达更深的位置,而不会被波长的生物组织上层吸收, 在组织工程中命名为 “治疗窗”。不幸的是,快速消除和在水介质中的短期稳定性限制了其作为早期检测癌组织的荧光探针的使用。在这项研究中,ICG 的稳定是通过将 ICG 分子封装到与聚 (l-乳酸) 和聚 (ε-己内酯) 复合的生物可降解聚合物中进行的。通过简单的一步多轴静电纺丝方法。进行了不同类型的同轴和三轴结构基团,并与单一的仅聚合物基团进行了比较。用共聚焦显微镜对电纺纳米纤维内包裹的 ICG (1 mg/mL) 进行成像,并在体外对 MIA PaCa-2 胰腺癌细胞摄取 ICG。包裹 ICG 的稳定性通过 PBS (ph = 4 和 7) 中长达 21 天的体外可持续释放曲线得到证明。这些结果提示了 ICG 从体外植入 ICG 包裹的多轴纳米纤维垫内化和调节进入胰腺细胞胞浆的能力的潜力。ICG 封装的多层纳米纤维可能有望用于局部持续递送系统,以消除系统给药中直接注射负载 ICG 的纳米颗粒引起的剂量损失。

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影响因子:2.69
发表时间:2020-01-18
DOI:10.1016/j.bbrc.2020.01.048
作者列表:["Li Y","Wang Z","Jin J","Zhu SX","He GQ","Li SH","Wang J","Cai Y"]

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影响因子:2.46
发表时间:2020-01-01
DOI:10.1097/COC.0000000000000609
作者列表:["Appelt AL","Andersen RF","Lindebjerg J","Jakobsen A"]

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