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Scrophularia buergeriana attenuates allergic inflammation by reducing NF-κB activation.

补骨草通过减少 NF-κ b 活化减轻过敏性炎症。

  • 影响因子:4.09
  • DOI:10.1016/j.phymed.2019.153159
  • 作者列表:"Shin NR","Lee AY","Song JH","Yang S","Park I","Lim JO","Jung TY","Ko JW","Kim JC","Lim KS","Lee MY","Shin IS","Kim JS
  • 发表时间:2020-02-01
Abstract

BACKGROUND:Scrophularia buergeriana Miq. (Scrophulariaceae) (SB) has been used as an oriental medicine for the treatment of inflammatory diseases, such as neuritis and pharyngolaryngitis. PURPOSE:We explored the therapeutic effects of S. buergeriana ethanol extract (SBE) on airway inflammation in ovalbumin (OVA)-induced asthmatic mice and lipopolysaccharide (LPS)-stimulated RAW264.7 cells. METHODS:Mice were intraperitoneally injected with OVA on days 0 and 14 to elevate the immune response. On days 21 to 23, the mice were challenged with OVA solution and SBE (20 and 40 mg/kg) was administered daily by oral gavage from days 18 to 23. RAW264.7 cells were pretreated with SBE 1 h before LPS stimulation. RESULTS:SBE administration effectively suppressed inflammatory cell infiltration, the expression of interleukin (IL)-5, IL-13, and IL-17, immunoglobulin E, and airway hyperresponsiveness in an OVA-induced allergic asthma model. A reduction in histological alterations, including airway inflammation and mucus hypersecretion, was observed. These effects of SBE were accompanied by a decrease in matrix metalloproteinase-9 (MMP-9) expression and nuclear factor kappa B (NF-κB) phosphorylation. These responses were observed in LPS-stimulated RAW264.7 cells. SBE treatment reduced the mRNA expression of tumor necrosis factor (TNF)-α, IL-6, and MMP-9, and NF-κB phosphorylation, in LPS-stimulated RAW264.7 cells. CONCLUSION:Our results indicated that SBE effectively attenuated airway inflammation in an OVA-induced allergic asthma model. These properties of SBE were thought to be involved in the suppression of NF-κB phosphorylation, suggesting that the material has the potential to regulate the development of allergic asthma.

摘要

背景: 补骨草。(玄参科) (SB) 已作为东方医学用于治疗炎症性疾病,如神经炎和咽喉炎。 目的: 我们探讨了 buergeriana 乙醇提取物 (SBE) 对卵清蛋白 (OVA) 诱导的哮喘小鼠和脂多糖 (LPS) 刺激的 RAW264.7 细胞气道炎症的治疗作用。 方法: 在第 0 天和第 14 天,小鼠腹腔注射 OVA 以提高免疫反应。第 21-23 天,用 OVA 溶液攻击小鼠,第 18-23 天每天口服灌胃给予 SBE (20 和 40 mg/kg)。RAW264.7 细胞在 LPS 刺激前 1 h 用 SBE 预处理。 结果: 在 OVA 诱导的过敏性哮喘模型中,SBE 能有效抑制炎症细胞浸润、白细胞介素 (IL)-5 、 IL-13 和 IL-17 的表达、免疫球蛋白 E 和气道高反应性。观察到组织学改变减少,包括气道炎症和黏液高分泌。SBE 的这些作用伴随着基质 metalloproteinase-9 (MMP-9) 表达和核因子 κ B (NF-κ B) 磷酸化的减少。在 LPS 刺激的 RAW264.7 细胞中观察到这些反应。SBE 处理降低了 LPS 刺激的 RAW264.7 细胞中肿瘤坏死因子 (TNF)-α 、 IL-6 和 MMP-9 的 mRNA 表达以及 NF-κ b 磷酸化。 结论: 我们的研究结果表明,SBE 有效地减轻了 OVA 诱导的过敏性哮喘模型的气道炎症。SBE 的这些特性被认为参与抑制 NF-κ b 磷酸化,提示该物质具有调节过敏性哮喘发展的潜力。

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影响因子:3.94
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作者列表:["Bernstein DM","Toth B","Rogers RA","Kling DE","Kunzendorf P","Phillips JI","Ernst H"]

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影响因子:4.04
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DOI:10.1042/BST20191010
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