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The fluctuations of metabotropic glutamate receptor subtype 5 (mGluR5) in the Amygdala in fear conditioning model of male Wistar rats following sleep deprivation, reverse circadian and napping.
睡眠剥夺、反向昼夜节律和打盹后雄性 Wistar 大鼠恐惧条件反射模型杏仁核代谢型谷氨酸受体亚型 5 (mGluR5) 的波动。
- 影响因子:3.15
- DOI:10.1016/j.brainres.2020.146739
- 作者列表:"Kordestani-Moghadam P","Nasehi M","Khodagholi F","Vaseghi S","Zarrindast MR","Khani M
- 发表时间:2020-02-19
Abstract
:Sleep is involved in metabolic system, mental health and cognitive functions. Evidence shows that sleep deprivation (SD) negatively affects mental health and impairs cognitive functions, including learning and memory. Furthermore, the metabotropic glutamate receptor subtype 5 (mGluR5) is a metabolic biomarker, which is affected by various conditions, including stress, sleep deprivation, and cognitive and psychiatric disorders. In this research, we investigated the effect of SD and reverse circadian (RC), and two models of napping (continuous and non-continuous) combined with SD or RC on fear-conditioning memory, anxiety-like behavior and mGluR5 fluctuations in the amygdala. 64 male Wistar rats were used in this study. The water box apparatus was used to induce SD/RC for 48 hours, and fear-conditioning memory apparatus was used to assess fear memory. The results showed, fear-conditioning memory was impaired following SD and RC, especially in contextual stage. However, anxiety-like behavior was increased. Furthermore, mGluR5 was increased in the left amygdala more than the right amygdala. Additionally, continuous napping significantly improved fear-conditioning memory, especially freezing behavior. In conclusion, following SD and RC, fear-conditioning memory in contextual stage is more vulnerable than in auditory stage. Furthermore, increase in anxiety-like behavior is related to increase in the activity of left amygdala and mGluR5 receptors.
摘要
: 睡眠参与代谢系统、精神卫生和认知功能。有证据表明,睡眠剥夺 (SD) 对精神卫生产生负面影响,损害认知功能,包括学习和记忆。此外,代谢型谷氨酸受体亚型 5 (mGluR5) 是一种代谢生物标志物,受各种条件的影响,包括压力、睡眠剥夺以及认知和精神疾病。在这项研究中,我们调查了 SD 和反向昼夜节律 (RC),以及两种打盹模型 (连续和非连续) 与 SD 或 RC 结合对恐惧条件记忆的影响, 焦虑样行为和杏仁核 mGluR5 波动。本研究采用雄性 Wistar 大鼠 64 只。使用水箱装置诱导 SD/RC 48 小时,使用恐惧条件记忆装置评估恐惧记忆。结果显示,恐惧条件记忆在 SD 和 RC 之后受损,尤其是在情境阶段。然而,焦虑样行为增加。此外,mGluR5 在左侧杏仁核中的增加多于右侧杏仁核。此外,持续午睡显著改善了恐惧调节记忆,尤其是冻结行为。总之,遵循 SD 和 RC,情境阶段的恐惧条件记忆比听觉阶段更脆弱。此外,焦虑样行为的增加与左杏仁核和 mGluR5 受体活性的增加有关。
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