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Viral shedding, and distribution of cytomegalovirus glycoprotein H (UL75), glycoprotein B (UL55), and glycoprotein N (UL73) genotypes in congenital cytomegalovirus infection.

病毒脱落,先天性巨细胞病毒病毒感染巨细胞病毒病毒糖蛋白 H (UL75) 、糖蛋白 B (UL55) 和糖蛋白 N (UL73) 基因型的分布。

  • 影响因子:2.61
  • DOI:10.1016/j.jcv.2020.104287
  • 作者列表:"Puhakka L","Pati S","Lappalainen M","Lönnqvist T","Niemensivu R","Lindahl P","Nieminen T","Seuri R","Nupponen I","Boppana S","Saxen H
  • 发表时间:2020-02-11
Abstract

BACKGROUND:Children with congenital CMV infection (cCMV) shed virus in urine and saliva for prolonged periods of time. Outcome of cCMV varies from asymptomatic infection with no sequelae in most cases, to severe longterm morbidity. The factors associated with asymptomatic cCMV are not well defined. We evaluated the viral shedding in a cohort of infants with cCMV identified on newborn screening. In addition, we describe the distribution of viral genotypes in our cohort of asymptomatic infants and previous cohorts of cCMV children in the literature. METHODS:Study population consisted of 40 children with cCMV identified in screening of 19,868 infants, a prevalence of 2/1000. The viral shedding was evaluated at 3 and 18 months of age by real-time CMV-PCR of saliva and plasma, and CMV culture of urine. CMV positive saliva samples were analyzed for genotypes for CMV envelope glycoproteins gB (UL55), and gH (UL75) by genotype specific real-time PCR, and gN (UL73) by cloning and sequencing RESULTS: At 3 months age 40/40 saliva and urine samples, and 19/40 plasma samples were positive for CMV. At 18 months age all urine samples tested (33/33), 9/37 of saliva samples, and 2/34 plasma samples were positive for CMV. The genotype distribution did not differ from the published data. CONCLUSIONS: The urinary virus shedding is more persistent than salivary shedding in children with cCMV. The genotype distribution was similar to previous literature and does not explain the low disease burden of cCMV in our population.

摘要

背景: 先天性 CMV 感染 (cCMV) 患儿的尿液和唾液中病毒脱落时间较长。CCMV 的结局从大多数病例无后遗症的无症状感染到严重的长期发病率。与无症状 cCMV 相关的因素尚未明确。我们在新生儿筛查中发现的 cCMV 婴儿队列中评估了病毒脱落情况。此外,我们在文献中描述了我们的无症状婴儿队列和既往 cCMV 儿童队列中病毒基因型的分布。 方法: 研究人群由筛查 19,868 例婴儿中确定的 40 例 cCMV 患儿组成,患病率为 2/1000。通过唾液和血浆的实时 CMV-PCR 和尿液的 CMV 培养,在 3 个月和 18 个月时进行病毒脱落评价。CMV 阳性唾液样本通过基因型特异性实时 PCR 和 gN (UL73) 分析 CMV 包膜糖蛋白 gB (UL55) 和 gH (UL75) 的基因型通过克隆和测序结果: 3 月龄时 40/40 份唾液和尿液样本,19/40 份血浆样本 CMV 阳性。18 月龄时,所有检测的尿液样本 (33/33) 、 9/37 的唾液样本和 2/34 的血浆样本 CMV 阳性。基因型分布与已发表的数据无差异。 结论: cCMV 患儿尿病毒脱落较唾液脱落更持久。基因型分布与以往文献相似,不能解释我国人群 cCMV 的低疾病负担。

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