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Polymeric Films for the Encapsulation, Storage, and Tunable Release of Therapeutic Microbes.
用于治疗性微生物的包封、储存和可调释放的聚合物膜。
- 影响因子:5.97
- DOI:10.1002/adhm.201901643
- 作者列表:"Qiu K","Young I","Woodburn BM","Huang Y","Anselmo AC
- 发表时间:2020-02-20
Abstract
:Microbe-based therapeutics (MBTs) are an emerging therapeutic modality for treating gastrointestinal infections and inflammatory bowel diseases. Current formulations for oral delivery of MBTs use capsules to achieve safe gastric transit, but oral formulations that control the spatiotemporal concentration of MBTs are yet to be developed, despite well-established connections between all therapeutics and their location, concentration, and distribution at sites of action. The development of a multi-functional polymer-based encapsulation system to formulate MBTs for enhanced storage and delivery through formulation of a model MBT, Lactobacillus casei ATCC393, is reported here. This approach enables the additive inclusion of excipients and polymers to grant specific functions, toward the development of a modular MBT platform. Through addition of established excipients, the formulation provides long-term storage of the encapsulated MBT. By adding higher molecular weight polymers, the release kinetics of the encapsulated MBTs can be modified. The inclusion of a mucoadhesive polymer significantly increases the adhesion force between the formulation and the intestinal tissue. Together, mucoadhesive and sustained release properties can be used to modulate the spatiotemporal concentration of MBTs. The formulation is compatible with standard oral capsules, thus maintaining existing clinical advantages of oral capsules while providing new functions from film encapsulation.
摘要
: 基于微生物的治疗药物 (MBTs) 是治疗胃肠道感染和炎症性肠病的新兴治疗方式。目前用于口服递送 MBTs 的制剂使用胶囊来实现安全的胃转运,但是控制 MBTs 时空浓度的口服制剂尚未开发, 尽管所有治疗方法与其在作用部位的位置、浓度和分布之间建立了良好的联系。本文报道了一种基于多功能聚合物的包封系统的开发,通过配制模型 MBT -- 干酪乳杆菌 ATCC393 来配制用于增强储存和递送的 MBTs。这种方法使得赋形剂和聚合物的添加剂包含能够赋予特定的功能,朝着模块化 MBT 平台的发展。通过添加已建立的赋形剂,该制剂提供了封装的 MBT 的长期储存。通过添加较高分子量的聚合物,可以改变封装的 MBTs 的释放动力学。包含粘膜粘附聚合物显著增加了制剂和肠组织之间的粘附力。同时,粘膜粘附和缓释特性可用于调节 MBTs 的时空浓度。该配方与标准口服胶囊相容,从而保持了口服胶囊现有的临床优势,同时提供了来自薄膜封装的新功能。
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