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Curdione ameliorates bleomycin-induced pulmonary fibrosis by repressing TGF-β-induced fibroblast to myofibroblast differentiation.

莪术二酮通过抑制 TGF-β 诱导的成纤维细胞向肌成纤维细胞分化来改善博莱霉素诱导的肺纤维化。

  • 影响因子:3.68
  • DOI:10.1186/s12931-020-1300-y
  • 作者列表:"Liu P","Miao K","Zhang L","Mou Y","Xu Y","Xiong W","Yu J","Wang Y
  • 发表时间:2020-02-19
Abstract

BACKGROUND:Idiopathic pulmonary fibrosis (IPF) is a progressive and irreversible disease characterized by excessive fibroblast to myofibroblast differentiation with limited therapeutic options. Curdione, a sesquiterpene compound extracted from the essential oil of Curcuma aromatica Salisb, has anti-inflammatory and anti-tumor effects. However, the role of curdione in IPF is still unclear. METHODS:The effects of curdione were evaluated in a bleomycin (BLM)-induced pulmonary fibrosis mouse model. C57BL/6 mice were treated with BLM on day 0 by intratracheal injection and intraperitoneal administered curdione or vehicle. In vitro study, expression of fibrotic protein was examined and the transforming growth factor (TGF)-β-related signaling was evaluated in human pulmonary fibroblasts (HPFs) treated with curdione following TGF-β1 stimulation. RESULTS:Histological and immunofluorescent examination showed that curdione alleviated BLM-induced lung injury and fibrosis. Specifically, curdione significantly attenuated fibroblast to myofibroblast differentiation in the lung in BLM induced mice. Furthermore, curdione also decreased TGF-β1 induced fibroblast to myofibroblast differentiation in vitro, as evidenced by low expression of α-SMA, collagen 1 and fibronectin in a dose dependent manner. Mechanistically, curdione suppressed the phosphorylation of Smad3 following TGF-β1 treatment, thereby inhibiting fibroblast differentiation. CONCLUSIONS:Overall, curdione exerted therapeutic effects against pulmonary fibrosis via attenuating fibroblast to myofibroblast differentiation. As curdione had been shown to be safe and well-tolerated in BLM-induced mouse model, curdione might be useful for developing novel therapeutics for IPF.

摘要

背景: 特发性肺纤维化 (IPF) 是一种进展性和不可逆的疾病,其特征是成纤维细胞向肌成纤维细胞过度分化,治疗选择有限。莪术二酮是从郁金挥发油中提取的倍半萜类化合物,具有抗炎和抗肿瘤作用。然而,莪术二酮在 IPF 中的作用仍不清楚。 方法: 在博莱霉素 (BLM) 诱导的肺纤维化小鼠模型中评价莪术二酮的作用。C57BL/6 小鼠在第 0 天通过气管内注射和腹腔给予莪术二酮或溶剂给予 BLM。在体外研究中,检测 TGF-β 1 刺激后莪术二酮处理的人肺成纤维细胞 (HPFs) 中纤维化蛋白的表达,并评价转化生长因子 (TGF)-β 相关信号。 结果: 组织学和免疫荧光检测显示莪术二酮可减轻 BLM 诱导的肺损伤和纤维化。具体而言,莪术二酮显著减弱 BLM 诱导小鼠肺成纤维细胞向肌成纤维细胞的分化。此外,莪术二酮还能降低 tgf-β 1 诱导的成纤维细胞向肌成纤维细胞的体外分化,其证据是 α-SMA 、胶原 1 和纤维连接蛋白的低表达呈剂量依赖性。机制上,莪术二酮抑制 tgf-β 1 处理后 Smad3 的磷酸化,从而抑制成纤维细胞分化。 结论: 总体上,莪术二酮通过减弱成纤维细胞向肌成纤维细胞分化而发挥抗肺纤维化的治疗作用。由于莪术二酮在 BLM 诱导的小鼠模型中被证明是安全和耐受性良好的,因此莪术二酮可能有助于开发 IPF 的新型治疗药物。

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作者列表:["Vadillo C","Nieto MA","Romero-Bueno F","Leon L","Sanchez-Pernaute O","Rodriguez-Nieto MJ","Freites D","Jover JA","Álvarez-Sala JL","Abasolo L"]

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翻译标题与摘要 下载文献
影响因子:4.40
发表时间:2020-01-01
DOI:10.1007/s00262-019-02431-8
作者列表:["Shibaki, Ryota","Murakami, Shuji","Matsumoto, Yuji","Yoshida, Tatsuya","Goto, Yasushi","Kanda, Shintaro","Horinouchi, Hidehito","Fujiwara, Yutaka","Yamamoto, Nobuyuki","Kusumoto, Masahiko","Yamamoto, Noboru","Ohe, Yuichiro"]

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翻译标题与摘要 下载文献
影响因子:4.04
发表时间:2020-01-25
来源期刊:New biotechnology
DOI:10.1016/j.nbt.2019.08.006
作者列表:["Sousa SA","Soares-Castro P","Seixas AMM","Feliciano JR","Balugas B","Barreto C","Pereira L","Santos PM","Leitão JH"]

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