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Immunosensor incorporating half-antibody fragment for electrochemical monitoring of amyloid-β fibrils in artificial blood plasma.

掺入半抗体片段的免疫传感器用于人工血浆中淀粉样蛋白-β 原纤维的电化学监测。

  • 影响因子:4.37
  • DOI:10.1016/j.bioelechem.2020.107643
  • 作者列表:"Palla G","Malecka K","Dehaen W","Radecki J","Radecka H
  • 发表时间:2021-02-01
Abstract

:In this report, an electrochemical immunosensor for the selective and sensitive monitoring of Aβ1-42 fibrils is presented. The sensing platform was prepared by the formation of a 4,4'-thiobisbenzenethiol (TBBT) self-assembled monolayer on a clean gold surface followed by the covalent entrapment of gold nanoparticles (AuNPs). The half-antibody fragments of the Anti-Amyloid Fibrils antibody were immobilized on AuNPs via S-Au covalent bonds. Each step of immunosensor fabrication was characterized with cyclic voltammetry and electrochemical impedance spectroscopy. The biosensor was successfully used for the sensing of Aβ1-42 fibrils in both phosphate saline buffer (PBS) and artificial blood plasma (ABP). The immunosensor sensitivity estimated based on calibration slopes was better in the presence of APP in the comparison to PBS. The LOD values obtained for both measuring media were of 0.6 pM level. The moderate response towards Aβ1-42 oligomers demonstrated the immunosensor selectivity.

摘要

: 在本报告中,介绍了一种用于选择性和灵敏监测Aβ1-42原纤维的电化学免疫传感器。通过在干净的金表面上形成4,4 '-硫代双苯并硫醇 (TBBT) 自组装单层,然后共价截留金纳米颗粒 (AuNPs) 来制备传感平台。抗淀粉样原纤维抗体的半抗体片段通过S-Au共价键固定在aunp上。用循环伏安法和电化学阻抗谱对免疫传感器制备的各个步骤进行了表征。该生物传感器成功地用于感测磷酸盐盐水缓冲液 (PBS) 和人工血浆 (ABP) 中的Aβ1-42原纤维。与PBS相比,基于校准斜率估计的免疫传感器灵敏度在APP存在的情况下更好。两种测量培养基获得的LOD值均为0.6 pM水平。对Aβ1-42寡聚体的中等响应证明了免疫传感器的选择性。

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影响因子:2.68
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DOI:10.1080/14656566.2020.1814255
作者列表:["Sawada H","Oeda T","Kohsaka M","Tomita S","Umemura A","Park K","Yamamoto K","Kiyohara K"]

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DOI:10.1007/s11033-021-06299-9
作者列表:["Louvrier A","Terranova L","Meyer C","Meyer F","Euvrard E","Kroemer M","Rolin G"]

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