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Silver or gold? A comparison of nanoparticle modified electrochemical genosensors based on cobalt porphyrin-DNA.

银还是金?基于钴卟啉-DNA的纳米粒子修饰电化学基因传感器的比较。

  • 影响因子:4.37
  • DOI:10.1016/j.bioelechem.2020.107723
  • 作者列表:"Malecka K","Kaur B","Cristaldi DA","Chay CS","Mames I","Radecka H","Radecki J","Stulz E
  • 发表时间:2021-04-01
Abstract

:We applied a cobalt-porphyrin modified DNA as electrochemical marker, which was attached to nanoparticles, to detect specific DNA sequences. We compare the performance of gold and silver NPs in oligonucleotide sensors to determine if a change in metal will lead to either higher sensitivity or different selectivity, based on the redox behaviour of silver vs. gold. Surprisingly, we find that using either gold or silver NPs yields very similar overall performance. The electrochemical measurements of both types of sensors show the same redox behaviour which is dominated by the cobalt porphyrin, indicating that the electron pathway does not include the NP, but there is direct electron transfer between the porphyrin and the electrode. Both sensors show a linear response in the range of 5 × 10-17-1 × 10-16 M; the limit of detection (LOD) is 3.8 × 10-18 M for the AuNP sensor, and 5.0 × 10-18 M for the AgNP sensor, respectively, which corresponds to the detection of about 20-50 DNA molecules in the analyte. Overall, the silver system results in a better DNA economy and using cheaper starting materials for the NPs, thus shows better cost-effectivness and could be more suitable for the mass-production of highly sensitive DNA sensors.

摘要

: 我们应用钴卟啉修饰的DNA作为电化学标记,其附着于纳米颗粒,以检测特定的DNA序列。我们比较了金和银NPs在寡核苷酸传感器中的性能,以确定金属的变化是否会导致更高的灵敏度或不同的选择性,基于银与金的氧化还原行为。令人惊讶的是,我们发现使用金或银NPs产生非常相似的整体性能。两种类型的传感器的电化学测量显示由钴卟啉主导的相同的氧化还原行为,表明电子途径不包括NP,但是在卟啉和电极之间存在直接的电子转移。两种传感器均在5 × 10-17-1 × 10-16 M范围内呈现线性响应; AuNP传感器的检测限 (LOD) 分别为3.8 × 10-18 M,AgNP传感器为5.0 × 10-18 M,这对应于分析物中约20-50个DNA分子的检测。总的来说,银系统导致更好的DNA经济性,并使用更便宜的起始材料用于NPs,因此显示出更好的成本效益,并且可能更适合高灵敏度DNA传感器的大规模生产。

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