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Investigating the photodynamic efficacy of chlorin e6 by millisecond pulses in metastatic melanoma cells.

通过毫秒脉冲研究二氢chlor呤e6在转移性黑色素瘤细胞中的光动力功效。

  • 影响因子:4.37
  • DOI:10.1016/j.bioelechem.2020.107728
  • 作者列表:"Kulbacka J","Chodaczek G","Rossowska J","Szewczyk A","Saczko J","Bazylińska U
  • 发表时间:2021-04-01
Abstract

:Melanoma is considered the most aggressive type of skin cancer, still without effective treatment. Thus, alternative therapeutic methods are still in demand, and electroporation-supported photodynamic therapy (EP-PDT) of cancer cells seems a promising approach. New developments in EP-PDT aim at enhanced tumor selectivity and biocompatibility by applying a second-generation photosensitizer, i.e., Chlorin e6 (Ce6). We have verified the improved photodynamic effect of Ce6 on skin cancer melanoma (Me45) cells and control (CHO-K1) cells. In this study, we applied 1 or 5 pulses of 10 ms duration and assessed the EP-PDT effect with a variety of tests, such as singlet oxygen scavenger (ABMDMA) photooxidation, oxidoreductive potential measurements, kinetic measurements with fluorescent microscopy, photosensitizer uptake studies, lipid peroxidation level, and actin fibers organization. The optimization of photosensitizer uptake as a function of temperature was also performed. Our results indicated efficient Ce6 delivery into Me45 cells and good photodynamic efficiency enhanced by the electroporation of cancer cells.

摘要

: 黑色素瘤被认为是最具侵袭性的皮肤癌类型,仍然没有有效的治疗。因此,仍然需要替代的治疗方法,并且癌细胞的电穿孔支持的光动力疗法 (ep-pdt) 似乎是有希望的方法。Ep-pdt的新发展旨在通过应用第二代光敏剂 (即,Chlorin e6 (Ce6)。我们已经验证了Ce6对皮肤癌黑素瘤 (Me45) 细胞和对照 (CHO-K1) 细胞的改善的光动力作用。在这项研究中,我们应用了1或5个10毫秒持续时间的脉冲,并通过各种测试评估了EP-PDT效应,如单线态氧清除剂 (ABMDMA) 光氧化、氧化还原电位测量、荧光显微镜动力学测量、光敏剂摄取研究、脂质过氧化水平和肌动蛋白纤维组织。还进行了作为温度的函数的光敏剂吸收的优化。我们的结果表明,Ce6有效地递送到Me45细胞中,并且癌细胞的电穿孔增强了良好的光动力效率。

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