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Does open ovarian biopsy in prepubertal age affect ovarian reserve in a rat model?

青春期前卵巢开放活检是否影响大鼠模型的卵巢储备?

  • 影响因子:2.14
  • DOI:10.1016/j.jpedsurg.2020.05.046
  • 作者列表:"Łuczak J","Bagłaj M","Ciaputa R","Szymerowski A","Nowak M
  • 发表时间:2021-02-01
Abstract

BACKGROUND:Partial resection of the ovary carries a possible risk of fertility reduction. We studied the influence of open ovarian biopsy on ovarian reserve, including anti-Müllerian hormone and follicle-stimulating hormone serum level evaluation, in a prepubertal rat model. METHODS:Interventions - the initial surgery was unilateral ovarian biopsy (38 rats, group B1, B2) or unilateral ovarian biopsy and ovarian resection of the contralateral gonad (38 rats, group BR1, BR2). The second operation was bilateral ovarian resection and total resection of the remaining ovary. All rats had hormone serum levels evaluated. The control group had only a blood test taken and bilateral ovarian resection done at the second intervention (30 rats, group C1, C2). The collected tissue was examined estimating follicle count and anti-Müllerian hormone immunoexpression. RESULTS:Anti-Müllerian hormone levels were significantly lower at the second intervention in the group BR2 but significantly higher in the group C2. Follicle-stimulating hormone levels were significantly higher in all but one group (BR2). CONCLUSIONS:Biopsy itself might not reduce ovarian reserve if done properly but we should know its possible negative effects in the case of a single remaining ovary.

摘要

背景: 卵巢部分切除可能有降低生育能力的风险。我们在青春期前大鼠模型中研究了开放卵巢活检对卵巢储备的影响,包括抗苗勒管激素和卵泡刺激素血清水平评估。 方法: 干预-最初的手术是单侧卵巢活检 (38只大鼠,B1,B2组) 或单侧卵巢活检和对侧性腺的卵巢切除 (38只大鼠,BR1,BR2组)。第二次手术为双侧卵巢切除,剩余卵巢全切除。所有大鼠都评估了激素血清水平。对照组仅进行血液测试并在第二次干预时进行双侧卵巢切除术 (30只大鼠,组C1,C2)。检查收集的组织,估计卵泡计数和抗苗勒管激素免疫表达。 结果: BR2组第二次干预时抗苗勒管激素水平显著降低,而c2组显著升高。除一组 (BR2) 外,所有组的促卵泡激素水平均显著升高。 结论: 如果做的好,活检本身可能不会减少卵巢储备,但我们应该知道它在单个剩余卵巢的情况下可能的负面影响。

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