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Hay vs. haylage; forage type influences the equine urinary metabonome and faecal microbiota.
干草与海苔; 饲料类型影响马尿代谢组和粪便微生物群。
- 影响因子:1.86
- DOI:10.1111/evj.13456
- 作者列表:"Leng J","McNally S","Walton G","Swann J","Proudman C","Argo C","Emery S","La Ragione R","Eustace R
- 发表时间:2021-04-26
Abstract
BACKGROUND:Gut microbial communities are increasingly being linked to diseases in animals and humans. Obesity and its associated diseases are a concern for horse owners and veterinarians, and there is a growing interest in the link between diet, the intestinal microbiota and metabolic disease. OBJECTIVES:Assess the influence of long-term hay or haylage feeding on the microbiota and metabolomes of 20 Welsh mountain ponies. STUDY DESIGN:Longitudinal study. METHODS:Urine, faeces and blood were collected from 20 ponies on a monthly basis over a 13-month period. Urine and faeces were analysed using proton magnetic resonance (1 H NMR) spectroscopy and faecal bacterial DNA underwent 16S rRNA gene sequencing. RESULTS:Faecal bacterial community profiles were observed to be different for the two groups, with discriminant analysis identifying 102 bacterial groups (or operational taxonomic units, OTUs) that differed in relative abundance in accordance with forage type. Urinary metabolic profiles of the hay and haylage fed ponies were significantly different during 12 of the 13 months of the study. Notably, the urinary excretion of hippurate was greater in the hay fed ponies for the duration of the study, while ethyl-glucoside excretion was higher in the haylage fed ponies. MAIN LIMITATIONS:The study was undertaken over a 13-month period and both groups of ponies had access to pasture during the summer months. CONCLUSIONS:The data generated from this study, suggest that the choice of forage may have implications for the intestinal microbiota and metabolism of ponies and therefore, potentially their health status. Understanding the potential implication of feeding a particular type of forage will enable horse owners to make more informed choices with regard to feed, especially if their horse or pony is prone to weight gain.
摘要
背景: 肠道微生物群落越来越多地与动物和人类的疾病联系在一起。肥胖及其相关疾病是马主人和兽医关注的问题,并且人们对饮食,肠道微生物群和代谢疾病之间的联系越来越感兴趣。 目的: 评估长期干草或海兔饲养对20匹威尔士山地小马微生物群和代谢组的影响。 研究设计: 纵向研究。 方法: 在13个月的时间里,每月收集20匹小马的尿液、粪便和血液。使用质子磁共振 (1 hnmr) 光谱法分析尿液和粪便,并对粪便细菌DNA进行16srrna基因测序。 结果: 观察到两组粪便细菌群落谱不同,判别分析确定了102个细菌组 (或操作分类单位,OTUs),它们根据饲料类型的相对丰度不同。在研究的13个月中的12个月期间,干草和海狗喂养的小马的尿液代谢特征显著不同。值得注意的是,在研究期间,干草喂养的小马中马尿酸盐的尿排泄较多,而乙基-葡萄糖苷排泄在海兔喂养的小马中较高。 主要限制: 该研究进行了13个月的时间,两组小马在夏季月份都可以进入牧场。 结论: 本研究产生的数据表明,饲料的选择可能对小马的肠道微生物群和代谢有影响,因此可能影响它们的健康状况。了解喂养特定类型饲料的潜在影响将使马主能够在饲料方面做出更明智的选择,特别是如果他们的马或小马容易体重增加。
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