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Effects of endurance racing on horse plasma extracellular particle miRNA.

耐力竞赛对马血浆胞外颗粒miRNA的影响。

  • 影响因子:1.86
  • DOI:10.1111/evj.13300
  • 作者列表:"de Oliveira GP Jr","Porto WF","Palu CC","Pereira LM","Reis AMM","Marçola TG","Teixeira-Neto AR","Franco OL","Pereira RW
  • 发表时间:2021-05-01
Abstract

BACKGROUND:Physical exercise is an essential factor in preventing and treating metabolic diseases by promoting systemic benefits throughout the body. The molecular factors involved in this process are poorly understood. Micro RNAs (miRNAs) are small non-coding RNAs that inhibit mRNA transcription. MiRNAs, which can participate in the benefits of exercise to health, circulate in plasma in extracellular particles (EP). Horses that undergo endurance racing are an excellent model to study the impact of long-duration/low intensity exercise in plasma EP miRNAs. OBJECTIVES:To evaluate the effects of 160 km endurance racing on horse plasma extracellular particles and their miRNA population. STUDY DESIGN:Cohort study. METHODS:We collected plasma from five Arabian horses during five time-points of an endurance ride. Extracellular particles were purified from plasma and characterised by electron microscopy, resistive pulse sensing (qNano) and western blotting. Small RNAs were purified from horse plasma EP, and sequencing was performed. RESULTS:Endurance racing increased EP concentration and average diameter compared to before the race. Western blotting showed a high concentration of extracellular vesicles proteins 2 hours after the race, which returned to baseline 15 hours after the race. MicroRNA differential expression analysis revealed increasing levels of eca-miR-486-5p during and after the race, and decreasing levels of eca-miR-9083 after the end. CONCLUSIONS:This study adds new data about the variation in plasma EP concentrations after long-distance exercise and brings new insights about the roles of exercise-derived EP miRNAs during low-intensity endurance exercise.

摘要

背景: 体育锻炼是预防和治疗代谢性疾病的重要因素,通过促进全身益处。这一过程中涉及的分子因素知之甚少。微rna (mirna) 是抑制mRNA转录的小的非编码rna。可以参与运动对健康的益处的mirna在胞外颗粒 (EP) 中的血浆中循环。经历耐力比赛的马是研究血浆EP mirna中的长时间/低强度运动的影响的优秀模型。 目的: 评估160千米耐力赛跑对马血浆细胞外颗粒及其miRNA群体的影响。 研究设计: 队列研究。 方法: 我们在耐力骑行的五个时间点收集了五匹阿拉伯马的血浆。从血浆中纯化细胞外颗粒,并通过电子显微镜、电阻脉冲传感 (qNano) 和蛋白质印迹进行表征。从马血浆EP中纯化小rna,并进行测序。 结果: 与比赛前相比,耐力比赛增加了EP浓度和平均直径。蛋白质印迹法在比赛后2小时显示高浓度的细胞外囊泡蛋白,其在比赛后15小时回到基线。MicroRNA差异表达分析显示在比赛期间和之后eca-miR-486-5p的水平增加,并且在结束之后eca-miR-9083的水平降低。 结论: 这项研究增加了关于长距离运动后血浆EP浓度变化的新数据,并带来了关于运动衍生的EP mirna在低强度耐力运动中的作用的新见解。

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