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A Safety Study of the Effects of 2-Dimensional Shear Wave Elastography on Synaptic Morphologic Characteristics and Function in the Hippocampus of Neonatal Mice.

二维剪切波弹性成像对新生小鼠海马突触形态特征和功能影响的安全性研究。

  • 影响因子:1.57
  • DOI:10.1002/jum.15387
  • 作者列表:"Zhang C","Li N","Li C","Li J
  • 发表时间:2021-01-01
Abstract

OBJECTIVES:The aim of this study was to determine the effects of 2-dimensional (2D) shear wave elastography (SWE) on synaptic morphologic characteristics and function in the neonatal mouse hippocampus and whether it affects the capacity for learning and memory later in life. METHODS:We divided neonatal mice into a control group and a 2D SWE group scanned for 10, 20, or 30 minutes. Hippocampal morphologic characteristics were assessed by hematoxylin-eosin and Nissl staining. Ultrastructures of hippocampal neurons were visualized by electron microscopy. Protein and messenger RNA expression levels of synaptophysin, N-methyl-d-aspartate receptor 1 (NMDAR1), NMDAR2A, and NMDAR2B were quantified by a western blot and polymerase chain reaction, respectively. Learning and memory of adult mice were evaluated by the Morris water maze and the novel object recognition task. RESULTS:Compared with the control group, the hippocampal morphologic characteristics of the experimental groups did not differ under light microscopy, and the synaptic structures assessed by electron microscopy appeared normal. Western blot and polymerase chain reaction results showed that expression of synaptophysin, NMDAR1, NMDAR2A, and NMDAR2B were downregulated after exposure to 2D SWE, but there were no statistical differences between the experimental groups. This downregulation disappeared within 24 hours. The results of the Morris water maze and novel object recognition suggested that the 2D SWE scanning on neonatal mice had no effect on learning and memory in adulthood. CONCLUSIONS:This study demonstrated that when the mice were exposed to neonatal cranial ultrasound by 2D SWE lasting for longer than 10 minutes, the expression of genes involved in synaptic function was affected, but this effect lasted no longer than 24 hours and did not affect learning and memory in adulthood.

摘要

目的: 本研究的目的是确定二维 (2D) 剪切波弹性成像 (SWE) 对新生小鼠海马突触形态特征和功能的影响,以及它是否影响以后的学习和记忆能力。 方法: 我们将新生小鼠分为对照组和2D SWE组,扫描10、20或30分钟。通过苏木精-伊红和尼氏染色评估海马形态特征。通过电子显微镜观察海马神经元的超微结构。通过蛋白质印迹和聚合酶链反应分别定量突触素、N-甲基-d-天冬氨酸受体1 (NMDAR1) 、NMDAR2A和NMDAR2B的蛋白质和信使RNA表达水平。通过Morris水迷宫和新物体识别任务评价成年小鼠的学习和记忆。 结果: 与对照组相比,光镜下实验组大鼠海马形态特征无差异,电镜下突触结构表现正常。Western blot和聚合酶链反应结果显示,暴露于2D SWE后,突触素、NMDAR1、NMDAR2A和NMDAR2B的表达下调,但各实验组之间无统计学差异。这种下调在24小时内消失。Morris水迷宫和新物体识别结果表明,新生小鼠2D SWE扫描对成年后学习记忆无影响。 结论: 本研究表明,当小鼠暴露于新生儿颅脑超声持续超过10分钟时,突触功能相关基因的表达受到影响,但这种影响持续时间不超过24小时,并且不影响成年后的学习和记忆。

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