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Wing: A suitable nonlethal tissue type for repeatable and rapid telomere length estimates in bats.

Wing: 蝙蝠端粒长度可重复快速估计的合适非致死组织类型。

  • 影响因子:7.01
  • DOI:10.1111/1755-0998.13276
  • 作者列表:"Power ML","Power S","Bertelsen MF","Jones G","Teeling EC
  • 发表时间:2021-02-01
Abstract

:Telomeres are used increasingly in ecology and evolution as biomarkers for ageing and environmental stress, and are typically measured from DNA extracted from nonlethally sampled blood. However, obtaining blood is not always possible in field conditions and only limited amounts can be taken from small mammals, such as bats, which moreover lack nucleated red blood cells and hence yield relatively low amounts of DNA. As telomere length can vary within species according to age and tissue, it is important to determine which tissues serve best as a representation of the organism as a whole. Here, we investigated whether wing tissue biopsies, a rapid and relatively noninvasive tissue collection method, could serve as a proxy for other tissues when measuring relative telomere length (rTL) in the Egyptian fruit bat (Rousettus aegyptiacus). Telomeres were measured from blood, brain, heart, kidney, liver lung, muscle and wing, and multiple wing biopsies were taken from the same individuals to determine intra-individual repeatability of rTL measured by using qPCR. Wing rTL correlated with rTL estimates from most tissues apart from blood. Blood rTL was not significantly correlated with rTL from any other tissue. Blood and muscle rTLs were significantly longer compared with other tissues, while lung displayed the shortest rTLs. Individual repeatability of rTL measures from wing tissue was high (>70%). Here we show the relationships between tissue telomere dynamics for the first time in a bat, and our results provide support for the use of wing tissue for rTL measurements.

摘要

: 端粒在生态学和进化中越来越多地用作衰老和环境压力的生物标志物,并且通常从从非致死取样的血液中提取的DNA中测量。然而,在野外条件下获得血液并不总是可能的,并且只能从小型哺乳动物 (例如蝙蝠) 获得有限的量,这些哺乳动物此外缺乏有核红细胞,因此产生相对较低量的DNA。由于端粒长度可以根据年龄和组织在物种内变化,因此确定哪些组织最适合作为生物体整体的代表是重要的。在这里,我们研究了在测量埃及果蝠 (Rousettus aegyptiacus) 的相对端粒长度 (rTL) 时,翼组织活检 (一种快速且相对无创的组织采集方法) 是否可以作为其他组织的代理。从血液、脑、心脏、肾、肝、肺、肌肉和翼测量端粒,并且从相同个体获取多个翼活检以确定通过使用qPCR测量的rTL的个体内可重复性。Wing rTL与来自除血液之外的大多数组织的rTL估计值相关。血液rTL与来自任何其他组织的rTL不显著相关。与其他组织相比,血液和肌肉rTLs显著更长,而肺显示最短的rTLs。来自翼组织的rTL测量的个体可重复性高 (>70%)。在这里,我们首次在蝙蝠中展示了组织端粒动力学之间的关系,我们的结果为使用翼组织进行rTL测量提供了支持。

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